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In situ simultaneous profiling of phosphorylation and ubiquitination by single excitation-duplexed luminescence resonance energy transfer†
Chemical Communications ( IF 4.9 ) Pub Date : 2018-03-17 00:00:00 , DOI: 10.1039/c8cc01494a
Fengjiao Ma 1, 2, 3, 4, 5 , Lei Zhang 1, 2, 3, 4, 5 , Bernardino J. Córdova Wong 1, 2, 3, 4, 5 , Jianping Lei 1, 2, 3, 4, 5
Affiliation  

A dual luminescence resonance energy transfer system is developed based on aptamer-functionalized upconversion nanoparticles as the energy donor and a synthetic dye derivative as the acceptor, which can be applied in the simultaneous profiling and relative quantification of phosphorylation and ubiquitination on a specific protein, even at low levels of protein expression.

中文翻译:

通过单激发双工发光共振能量转移在原位同时进行磷酸化和泛素化分析

基于适体功能化的上转换纳米粒子作为能量供体,合成染料衍生物作为受体,开发了一种双发光共振能量转移系统,该系统可用于同时对特定蛋白质甚至磷酸化和泛素化进行分析和相对定量低水平的蛋白质表达。
更新日期:2018-03-17
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