Sensitive detection of Hepatitis C virus (HCV) DNA is critical to treat and prevent the propagation of hepatitis C diseases. Herein a FRET probe of One-Donor-Multi-Acceptors (D–mA) assembly is designed through the formation of triple-helix DNA structure, which can greatly reduce the background of FRET system so as to greatly increase the detection sensitivity of hepatitis C virus DNA with the limit of detection (3σ) being 24.57 nmol/L, making the detection of clinic samples successful and showing that the newly developed FRET probe of D–mA assembly could be applied for the early diagnosis and treatment of hepatitis C disease.

中文翻译：

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使用一供四受体FRET探针高灵敏度检测丙型肝炎病毒DNA

敏感地检测丙型肝炎病毒（HCV）DNA对于治疗和预防丙型肝炎疾病的传播至关重要。本文通过三螺旋DNA结构的形成设计了单供体多受体（D-mA）装配体的FRET探针，可以大大降低FRET系统的背景，从而大大提高丙型肝炎的检测灵敏度。病毒DNA的检出限（3σ）为24.57 nmol / L，使临床样品的检测成功，并且表明新开发的D–mA组件FRET探针可用于丙型肝炎疾病的早期诊断和治疗。