In the last years, the agricultural expansion has led to an increased use of pesticides, with glyphosate as the most widely used worldwide. This is also the situation in Argentina, where glyphosate formulations are the most commercialized herbicides. It is known that glyphosate formulations are much more toxic than the active ingredient, and this difference in toxicity can be attributed to the adjuvants present in the formula. In this context, the aim of the present study was to evaluate and compare sub-lethal histological effects of the glyphosate formulation Roundup Ultramax and glyphosate active ingredient on Leptodactylus latrans tadpoles at Gosner-stage 36. Semi-static bioassays were performed using 96 h of exposure with Roundup Ultramax formulation (RU; 0.37-5.25 mg a.e./L), glyphosate (GLY; 3-300 mg/L), and a control group. RU exposure showed an increment in the melanomacrophagic cells (MMc) and melanomacrophagic centers (MMCs) from 0.37 mg a.e./L. GLY exposure showed a significant increment in the number of MMc from 15 mg/L, and of MMCs from 3 mg/L. Also, histopathological lesions were observed in the liver of tadpoles exposed to both, GLY and RU. These lesions included: lipidosis and hepatic congestion, but only RU showed significant differences respect to control, with a LOEC value of 2.22 mg a.e./L for both effects. In sum, this study represents the first evidence of adverse effects of glyphosate and RU formulation on the liver of anuran larvae at concentrations frequently found in the environment.

在过去的几年中，农业的发展导致农药的使用增加，草甘膦是世界上使用最广泛的农药。在阿根廷，草甘膦制剂是最商业化的除草剂，阿根廷的情况也是如此。已知草甘膦制剂的毒性比活性成分大得多，这种毒性差异可归因于该制剂中存在的佐剂。在这种情况下，本研究的目的是评估和比较草甘膦制剂Roundup Ultramax和草甘膦活性成分对Latransodactylus latrans的亚致死性组织学影响。Go在Gosner阶段36。半静态生物测定法是使用Roundup Ultramax制剂（RU; 0.37-5.25 mg ae / L），草甘膦（GLY; 3-300 mg / L）和对照组在96小时的暴露下进行的。RU暴露表明，黑色素吞噬细胞（MMc）和黑色素吞噬中心（MMC）从0.37 mg ae / L增加。GLY暴露显示MMc的数量从15 mg / L显着增加，MMCs的数量从3 mg / L显着增加。而且，在暴露于GLY和RU的t的肝脏中观察到组织病理学损害。这些病变包括：脂质增生和肝充血，但只有RU与对照相比显示出显着差异，两种作用的LOEC值均为2.22 mg ae / L。总共，