Lung cancer is the second most prevalent cancer. Nonsmall cell lung cancer (NSCLC) is the most common type of lung cancer. The low efficacy in current chemotherapies impels us to find new alternatives to prevent or treat NSCLC. Rice bran oil is cytotoxic to A549 cells, a NSCLC cell line. Here, we identified 24-methylenecyloartanyl ferulate (24-mCAF) as the main component responsible for the cytotoxicity in A549 cells. An iTRAQ-based quantitative proteomics analysis revealed that 24-mCAF inhibits cell proliferation and activates cell death and apoptosis. 24-mCAF induces up-regulation of Myb binding protein 1A (MYBBP1A), a tumor suppressor that halts cancer progression. 24-mCAF inhibits the activity of AKT and Aurora B kinase, two Ser/Thr kinases involved in MYBBP1A regulation and that represent important targets in NSCLC. This study provides the first insight of the effect of 24-mCAF, the main component of rice bran oil, on A459 cells at the cellular and molecular levels.

中文翻译：

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通过MYBBP1A上调以及AKT和Aurora B激酶抑制作用，阿魏酸24-亚甲基环戊烷酯对A549非小细胞肺癌细胞的细胞毒性作用。

肺癌是第二大流行癌症。非小细胞肺癌（NSCLC）是最常见的肺癌类型。当前化学疗法的低效率促使我们寻找预防或治疗NSCLC的新替代方法。米糠油对NSCLC细胞系A549细胞具有细胞毒性。在这里，我们确定了24-mCAF阿魏酸亚甲基环戊烷酯是负责A549细胞细胞毒性的主要成分。基于iTRAQ的定量蛋白质组学分析显示24-mCAF抑制细胞增殖并激活细胞死亡和凋亡。24-mCAF诱导Myb结合蛋白1A（MYBBP1A）的上调，这是一种阻止癌症进展的肿瘤抑制因子。24-mCAF抑制AKT和Aurora B激酶（参与MYBBP1A调节的两个Ser / Thr激酶）的活性，这是NSCLC中的重要靶标。