Signal transduction by small ubiquitin-like modifier (SUMO) regulates a myriad of nuclear processes. Here we report on the role of SUMO in mitosis in human cell lines. Knocking down the SUMO conjugation machinery results in a delay in mitosis and defects in mitotic chromosome separation. Searching for relevant SUMOylated proteins in mitosis, we identify the anaphase-promoting complex/cyclosome (APC/C), a master regulator of metaphase to anaphase transition. The APC4 subunit is the major SUMO target in the complex, containing SUMO acceptor lysines at positions 772 and 798. SUMOylation is crucial for accurate progression of cells through mitosis and increases APC/C ubiquitylation activity toward a subset of its targets, including the newly identified target KIF18B. Combined, our findings demonstrate the importance of SUMO signal transduction for genome integrity during mitotic progression and reveal how SUMO and ubiquitin cooperate to drive mitosis.

中文翻译：

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SUMO的目标是APC / C，以调节从中期到后期的过渡。

小型泛素样修饰剂（SUMO）进行的信号转导调节了无数的核过程。在这里我们报告相扑在人类细胞系中有丝分裂中的作用。撞倒SUMO偶联机制会导致有丝分裂的延迟和有丝分裂染色体分离的缺陷。在有丝分裂中寻找相关的SUMO酰化蛋白，我们确定了后期促进复合物/环体（APC / C），这是中期到后期过渡的主要调控因子。APC4亚基是该复合物中的主要SUMO靶标，在772和798位包含SUMO受体赖氨酸。SUMO酰化对于细胞通过有丝分裂的准确进程至关重要，并提高APC / C泛素化活性朝着其靶标的一个子集，包括新近鉴定目标KIF18B。结合起来