The study comparatively evaluated diverse strategic models of cyclodextrin (CD) production by the CGTase of Bacillus firmus strain 37: continuous production and repetitive batches in ultrafiltration systems; immobilization of CGTase on curdlan and vegetable sponge natural supports; the use of the glycyrrhizin complexing agent to modulate CGTase selectivity in favor of γ-CD production. All strategies had in common the possibility of separation of CGTase from its inhibitory products and its reuse. In the continuous production model, at 48 h of assay, the highest productivity and selectivity for β-CD were obtained, 1.47 mmol/L/h and 92.8%, respectively. Glycyrrhizin was able to modulate the production of γ-CD with selectivity of 61.2% for 30-h batches. The comparative evaluation of the different strategic models for obtaining CDs showed particularities that should be considered, and most of the models studied returned satisfactory yields as well as excellent selectivity.

这项研究比较了坚定芽孢杆菌CGTase产生环糊精（CD）的各种策略模型的评估。菌株37：在超滤系统中的连续生产和重复批次；将CGTase固定在柯德兰和植物海绵的天然支持物上; 使用甘草甜素络合剂调节CGTase选择性，有利于γ-CD的生产。所有策略都具有将CGTase从其抑制产物中分离出来并重新使用的可能性。在连续生产模型中，在48小时的测定中，获得了最高的β-CD生产率和选择性，分别为1.47 mmol / L / h和92.8％。甘草甜素能够以30个批次的61.2％的选择性调节γ-CD的产生。对获得CD的不同策略模型的比较评估显示了应该考虑的特殊性，并且所研究的大多数模型都返回了令人满意的产率以及出色的选择性。