To protect human health from the side effects of acrylamide in heat-processed food samples, simple analytical approaches are highly desired to determine low concentrations of acrylamide. In this study, a simple, rapid and accurate fluorescent sensor was developed for detection of acrylamide based on gold nanoparticles (AuNPs) and FAM-labeled double-stranded DNA (FAM-dsDNA). The sensing method was developed in a way to produce a remarkable fluorescence intensity difference in the absence and presence of acrylamide. In the presence of acrylamide, the single-stranded DNA (ssDNA) and acrylamide adduct is formed. So that, FAM-labeled complementary strand DNA (FAM-csDNA) is free in the environment and adsorbed on the surface of AuNPs and as a result, the FAM is quenched by the AuNPs. Under optimized conditions, the presented fluorescent analytical approach showed high selectivity toward acrylamide with a wide linear response, 1 × 10⁻⁷ M–0.05 M, and a limit of detection (LOD) of 1 × 10⁻⁸ M for acrylamide. This new method indicated excellent analytical performance for acrylamide detection in potato fries water extract samples with LOD of 0.5 × 10⁻⁶ M.

为了保护人类健康免受热处理食品样品中丙烯酰胺的副作用，非常需要简单的分析方法来确定低浓度的丙烯酰胺。在这项研究中，开发了一种基于金纳米颗粒（AuNPs）和FAM标记的双链DNA（FAM-dsDNA）的简单，快速，准确的荧光传感器，用于检测丙烯酰胺。在不存在和存在丙烯酰胺的情况下，以产生明显荧光强度差异的方式开发了传感方法。在丙烯酰胺存在下，形成单链DNA（ssDNA）和丙烯酰胺加合物。因此，FAM标记的互补链DNA（FAM-csDNA）在环境中是自由的，并吸附在AuNPs的表面上，因此，FAM被AuNPs淬灭。在优化条件下，^-7  M–0.05 M， 丙烯酰胺的检出限（LOD）为1×10 ^-8M。该新方法显示出LOD为0.5×10 ^-6  M的马铃薯薯条水提取物样品中丙烯酰胺检测的优异分析性能。