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Ultrasensitive detection of miRNA-155 using multi-walled carbon nanotube-gold nanocomposites as a novel fluorescence quenching platform
Sensors and Actuators B: Chemical ( IF 8.4 ) Pub Date : 2018-03-15 , DOI: 10.1016/j.snb.2018.03.071
Haiyan Ma , Ning Xue , Zongbing Li , Ke Xing , Xiangmin Miao

Multi-walled carbon nanotube-gold nanocomposites (MWCNT/AuNCs) were prepared and used as a novel fluorescence quenching platform for ultrasensitive detection of miRNA-155. In the designed system, fluorophore labeled DNA probe was introduced, and the fluorescence signal of them were effectively quenched due to the adsorption of them on MWCNT/AuNCs. Upon the addition of target miRNA-155, DNA-RNA heteroduplexes were formed and desorbed from MWCNT/AuNCs to yield a strong fluorescence signal. In the presence of duplex-specific nuclease (DSN), DNA-RNA heteroduplexes became a substrate for the enzymatic hydrolysis of DNA strands and triggered cyclic signal amplification. Under optimal conditions, MWCNT/AuNCs displayed 93.2% of the quenching efficiency to the fluorophore labeled DNA probe, paving the way for ultrasensitive miRNA-155 detection with a detection limit down to 33.4 fM. Meantime, the present strategy showed excellent selectivity for miRNA-155 detection against other miRNAs including miRNA-21, miRNA-141, and mutated miRNA-155 strands. Moreover, satisfactory results were obtained for miRNA-155 detection in human serum samples, illustrating that the proposed sensing platform is applicable in disease diagnosis.



中文翻译:

使用多壁碳纳米管-金纳米复合物作为新型荧光猝灭平台进行miRNA-155的超灵敏检测

制备了多壁碳纳米管-金纳米复合材料(MWCNT / AuNCs),并将其用作用于miRNA-155超灵敏检测的新型荧光猝灭平台。在设计的系统中,引入了荧光团标记的DNA探针,由于它们在MWCNT / AuNCs上的吸附,荧光信号被有效地猝灭。加入靶标miRNA-155后,DNA-RNA异源双链形成并从MWCNT / AuNCs解吸,产生强荧光信号。在双链体特异性核酸酶(DSN)的存在下,DNA-RNA异源双链体成为DNA链酶促水解并触发循环信号放大的底物。在最佳条件下,MWCNT / AuNC对荧光团标记的DNA探针显示出93.2%的淬灭效率,为检测灵敏度低至33.4 fM的超灵敏miRNA-155检测铺平了道路。同时,本策略显示了针对miRNA-155检测针对其他miRNA(包括miRNA-21,miRNA-141和突变的miRNA-155链)的出色选择性。此外,在人血清样本中的miRNA-155检测中获得了令人满意的结果,说明所提出的传感平台可用于疾病诊断。

更新日期:2018-03-15
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