AbstractAmphiphilic C3-symmetric tris-ureas self-assemble into supramolecular hydrogels in aqueous solution. These supramolecular hydrogels were used as matrices for the electrophoresis of biopolymers such as proteins and nucleic acids. A unique separation mode in comparison to that of typical sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) was found during the electrophoresis of denatured proteins. Native proteins were separated on the basis of their isoelectric points and retained their activities. Affinity electrophoresis was realized by exploiting interactions between gelator glucosides and carbohydrate-binding proteins. Protein samples were efficiently recovered through an extremely simple operation, and up to 50% of the protein was extracted by centrifugation, which is a remarkable feature of electrophoresis using supramolecular hydrogels. Large DNA fragments that previously had been separated only by pulsed-field gel electrophoresis were separated using a supramolecular hydrogel matrix and a typical continuous-field electrophoresis apparatus. In this focus review, the author summarizes the electrophoresis of proteins and nucleic acids using our developed supramolecular hydrogel matrix.Amphiphilic C3-symmetric tris-ureas self-assemble into supramolecular hydrogels in aqueous solution. These supramolecular hydrogels were used as matrices for the electrophoresis of biopolymers (SUGE: SupramolecularGelElectrophoresis). A unique separation mode in comparison to that of SDS–PAGE was found during the electrophoresis of denatured proteins. Native proteins were separated on the basis of their isoelectric points and retained their activities. Large DNA fragments that previously had been separated only by pulsed-field gel electrophoresis were separated using a supramolecular hydrogel matrix and a typical continuous-field electrophoresis apparatus.

中文翻译：

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超分子凝胶电泳

摘要两亲性 C3 对称三脲在水溶液中自组装成超分子水凝胶。这些超分子水凝胶被用作生物聚合物（如蛋白质和核酸）电泳的基质。与典型的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 (SDS-PAGE) 相比，在变性蛋白质的电泳过程中发现了一种独特的分离模式。天然蛋白质根据其等电点进行分离并保留其活性。亲和电泳是通过利用凝胶糖苷和碳水化合物结合蛋白之间的相互作用来实现的。通过极其简单的操作有效回收蛋白质样品，通过离心提取高达 50% 的蛋白质，这是使用超分子水凝胶电泳的显着特征。使用超分子水凝胶基质和典型的连续场电泳装置分离以前仅通过脉冲场凝胶电泳分离的大 DNA 片段。在这篇重点综述中，作者总结了使用我们开发的超分子水凝胶基质进行蛋白质和核酸的电泳。两亲性 C3 对称三脲在水溶液中自组装成超分子水凝胶。这些超分子水凝胶用作生物聚合物电泳的基质（SUGE：SupramolecularGelElectrophoresis）。与 SDS-PAGE 相比，在变性蛋白质的电泳过程中发现了一种独特的分离模式。天然蛋白质根据其等电点进行分离并保留其活性。使用超分子水凝胶基质和典型的连续场电泳装置分离以前仅通过脉冲场凝胶电泳分离的大 DNA 片段。