The exploration of volatile organic compounds (VOCs) produced by cell lines may be a powerful and non-invasive tool for the study of the health risk of human exposure to atmospheric particulate matter (PM). In this work, we developed a sensitive solid phase microextraction-gas chromatography-mass spectrometry method (SPME-GC-MS) to analyze VOCs in breathed gas of PM2.5-induced human embryonic fibroblast cell line (MRC-5). A novel graphene oxide/polyaniline/polydopamine (GO/PANI/PDA) coating was prepared on a stainless steel wire via electrochemical deposition and self-polymerization for the first time. The GO/PANI/PDA coating exhibited high extraction efficiency, good thermal stability (> 380 ℃), excellent mechanical stability as well as long service time (> 150 times). Parameters that may affect the results were optimized systematically. Under the optimal conditions, VOCs including benzene series, aldehydes and alkane were detected with low limit of detection (0.2–2.0 μg L⁻¹) and good correlation (correlation coefficients above 0.9922). The relative standard deviations of within-day and between-day were 1.1–8.4% and 0.2–11.2%, respectively. Satisfactory recoveries of 82–117% indicated good repeatability of the method. The method has been successfully applied for the determination of target VOCs in the headspace gas of PM2.5-induced MRC-5 cell. And it is expected to provide an alternative tool for the study of cytotoxicology of atmospheric particulates.

对细胞系产生的挥发性有机化合物（VOC）的探索可能是研究人类暴露于大气颗粒物（PM）的健康风险的有力且非侵入性的工具。在这项工作中，我们开发了一种灵敏的固相微萃取-气相色谱-质谱法（SPME-GC-MS），用于分析PM2.5诱导的人类胚胎成纤维细胞系（MRC-5）的呼吸气体中的VOC。首次通过电化学沉积和自聚合在不锈钢线上制备了新型氧化石墨烯/聚苯胺/聚多巴胺（GO / PANI / PDA）涂层。GO / PANI / PDA涂层具有较高的萃取效率，良好的热稳定性（> 380℃），优异的机械稳定性以及较长的使用寿命（> 150倍）。系统地优化了可能影响结果的参数。^-1）和良好的相关性（相关系数高于0.9922）。日内和日间的相对标准偏差分别为1.1–8.4％和0.2–11.2％。82-117％的满意回收率表明该方法具有良好的重复性。该方法已成功应用于测定PM2.5诱导的MRC-5细胞顶空气体中的目标VOC。并且有望为研究大气颗粒的细胞毒理学提供一种替代工具。