A chiral ligand exchange capillary electrochromatography (CLE-CEC) protocol was designed and implemented for d,l-amino acids enantioseparation with poly(maleic anhydride-styrene-methacryloyl-l-arginine methyl ester) as the coating. The block copolymer was synthesized through the reversible addition fragmentation chain transfer reaction. In the constructed CLE-CEC system, poly (methacryloyl-l-arginine methyl ester) moiety of the block copolymer played the role as the immobilized chiral ligand and Zn (II) was used as the central ion. Key factors, including pH of buffer solution, ratio of Zn (II) to ligands, the mass ratio of monomers in the block copolymer, which affect the enantioresolution were investigated. Comparing with the bare capillary, the CLE-CEC enantioresolution was enhanced greatly with the coating one. 5 Pairs of d,l-amino acids enantiomers obtained baseline separation with 5 pairs partly separated. The mechanism of enhancement enantioresolution of the developed CLE-CEC system was explored briefly. Further, good linearities were achieved in the range of 25.0 μM–5.0 mM for quantitative analysis of d-glutamine (r² = 0.997) and l-glutamine (r² = 0.991). Moreover, the proposed CLE-CEC assay was successfully applied in the kinetics study of glutaminase by using l-glutamine as the substrate.

设计并实施了以聚（马来酸酐-苯乙烯-甲基丙烯酰基-1-精氨酸甲酯）为涂层的d，1-氨基酸对映体分离的手性配体交换毛细管电色谱法（CLE-CEC）方案。通过可逆的加成断裂链转移反应合成了嵌段共聚物。在构建的CLE-CEC系统，聚（甲基丙烯酰升嵌段共聚物的-精氨酸甲酯）部分起了固定化手性配体的作用，并且将Zn（II）用作中心离子。研究了影响缓冲液pH值，Zn（II）与配体的比例，嵌段共聚物中单体的质量比等对映体拆分的影响因素。与裸毛细管相比，涂层CLE-CEC对映体分辨率大大提高。获得5对d，l-氨基酸对映异构体的基线分离结果，其中5对部分分离。简要探讨了开发的CLE-CEC系统增强对映体的机理。此外，在d-谷氨酰胺（r ^2）的定量分析中，在25.0 μM–5.0 mM的范围内实现了良好的线性。 ＝ 0.997）和1-谷氨酰胺（r ²  ＝ 0.991）。此外，所提出的CLE-CEC测定法以1-谷氨酰胺为底物成功地用于谷氨酰胺酶的动力学研究。