Rapid and quantitative molecular diagnostics in the field, at home, and at remote clinics is essential for evidence-based disease management, control, and prevention. Conventional molecular diagnostics requires extensive sample preparation, relatively sophisticated instruments, and trained personnel, restricting its use to centralized laboratories. To overcome these limitations, we designed a simple, inexpensive, hand-held, smartphone-based mobile detection platform, dubbed “smart-connected cup” (SCC), for rapid, connected, and quantitative molecular diagnostics. Our platform combines bioluminescent assay in real-time and loop-mediated isothermal amplification (BART-LAMP) technology with smartphone-based detection, eliminating the need for an excitation source and optical filters that are essential in fluorescent-based detection. The incubation heating for the isothermal amplification is provided, electricity-free, with an exothermic chemical reaction, and incubation temperature is regulated with a phase change material. A custom Android App was developed for bioluminescent signal monitoring and analysis, target quantification, data sharing, and spatiotemporal mapping of disease. SCC’s utility is demonstrated by quantitative detection of Zika virus (ZIKV) in urine and saliva and HIV in blood within 45 min. We demonstrate SCC’s connectivity for disease spatiotemporal mapping with a custom-designed website. Such a smart- and connected-diagnostic system does not require any lab facilities and is suitable for use at home, in the field, in the clinic, and particularly in resource-limited settings in the context of Internet of Medical Things (IoMT).

中文翻译：

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基于智能手机的分子诊断和时空疾病图谱移动检测平台

在野外，家庭和远程诊所进行快速定量的分子诊断对于基于证据的疾病管理，控制和预防至关重要。传统的分子诊断方法需要大量的样品制备，相对复杂的仪器和训练有素的人员，从而将其使用限制在中央实验室中。为了克服这些限制，我们设计了一个简单，便宜，手持式，基于智能手机的移动检测平台，称为“智能连接杯”（SCC），用于快速，连接和定量的分子诊断。我们的平台将实时生物发光测定和基于回路的等温扩增（BART-LAMP）技术与基于智能手机的检测相结合，从而消除了对基于荧光的检测必不可少的激发源和光学滤光片的需求。提供了用于等温扩增的孵育加热器，该加热器不带电，但发生放热的化学反应，并且孵育温度由相变材料调节。开发了一个定制的Android App，用于生物发光信号监视和分析，目标定量，数据共享以及疾病的时空映射。通过在45分钟内定量检测尿液，唾液中的Zika病毒（ZIKV）和血液中的HIV证明了SCC的效用。我们通过定制设计的网站演示了SCC在疾病时空映射方面的连通性。这样的智能诊断系统不需要任何实验室设施，并且适合在家庭，野外，诊所中使用，特别是在医疗物联网（IoMT）的资源有限的环境中使用。