Novel copper metal organic framework nanoparticles Cu-MOF-NPs (C1) were prepared via two simple alternative methods and confirmed by analytical characterization using mass, IR, Raman, XRD spectrum, HR-TEM and TGA-DSC. Mass spectroscopy revealed the molecular ion peak at 647 m/z for the monomeric unit structure n[Cu(AIP)₂(PIY)(H₂O)₂]·4H₂O, the presence of which was further supported by mass fragmentation. The Raman spectrum revealed two separate peaks corresponding to D and G bands of carbon in the structure of C1. Moreover, TGA-DSC showed the presence of CuO. XRD data were typically consistent with Raman and TGA-DSC data. In addition, HR-TEM revealed that the morphology of the C1 nanoparticles is uniform with well-distributed elliptical/spherical particles with a size range from 7 to 19 nm. The spectrophotometric and biological activity studies based on Cu-MOF-NPs were analyzed. The results indicated that Cu-MOF-NPs (C1) were successfully used as biosensors for the assessment of the triiodothyronine hormone (T3). The calibration plot was achieved over the concentration range of 40.0–100.0 ng dl⁻¹ T3 with limits of detection (LOD) and quantitation (LOQ) of 1.46 and 4.85 ng dl⁻¹, respectively, and a correlation coefficient (r) of 0.973. Moreover, the Cu-MOF-NPs (C1) show more enhanced biological activity against various pathogens (five strains of bacteria: Gram positive and Gram negative) when compared to an antibacterial agent and the effectiveness of Cu-MOF-NPs increases with increasing particle dose. The interactions of MOF-NPs (C1) with the biological targets were studied.

中文翻译：

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新型铜金属-有机骨架纳米粒子的简单合成：生物传感和生物学应用†

通过两种简单的替代方法制备了新型铜金属有机骨架纳米粒子Cu-MOF-NPs（C1），并通过质量，IR，拉曼，XRD光谱，HR-TEM和TGA-DSC的分析表征得到了证实。质谱显示单体单元结构n [Cu（AIP）₂（PIY）（H ₂ O）₂ ]·4H ₂ O的分子离子峰在647 m / z处，其存在进一步受到质量裂解的支持。拉曼光谱揭示了两个独立的峰，分别对应于C1结构中碳的D和G谱带。此外，TGA-DSC显示出CuO的存在。XRD数据通常与拉曼和TGA-DSC数据一致。此外，HR-TEM显示，C1纳米粒子的形态是均匀的，椭圆形/球形粒子分布均匀，尺寸范围为7至19 nm。分析了基于Cu-MOF-NPs的分光光度法和生物活性研究。结果表明，Cu-MOF-NPs（C1）已成功用作生物传感器，用于评估三碘甲状腺素（T3）。在40.0–100.0 ng dl ^-1 T3的浓度范围内获得标定图，检测限（LOD）和定量限（LOQ）分别为1.46和4.85 ng dl ^-1，相关系数（r）的0.973。此外，与抗菌剂相比，Cu-MOF-NPs（C1）对各种病原体（五种细菌：革兰氏阳性和革兰氏阴性）显示出更高的生物活性，并且Cu-MOF-NPs的有效性随着颗粒的增加而增加。剂量。研究了MOF-NPs（C1）与生物学靶标的相互作用。