Cell-sized vesicles have tremendous potential both as miniaturised pL reaction vessels and in bottom-up synthetic biology as chassis for artificial cells. In both these areas the introduction of light-responsive modules affords increased functionality, for example, to initiate enzymatic reactions in the vesicle interior with spatiotemporal control. Here we report a system composed of nested vesicles where the inner compartments act as phototransducers, responding to ultraviolet irradiation through diacetylene polymerisation-induced pore formation to initiate enzymatic reactions. The controlled release and hydrolysis of a fluorogenic β-galactosidase substrate in the external compartment is demonstrated, where the rate of reaction can be modulated by varying ultraviolet exposure time. Such cell-like nested microreactor structures could be utilised in fields from biocatalysis through to drug delivery.

中文翻译：

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嵌套囊泡反应器中的光触发酶促反应。

细胞大小的囊泡既有微型化的pL反应容器，又有自下而上的合成生物学作为人造细胞的底盘，具有巨大的潜力。在这两个区域中，光响应模块的引入提供了增加的功能，例如，通过时空控制来引发囊泡内部的酶促反应。在这里，我们报告了一个由巢状囊泡组成的系统，其中内部隔室起着光电换能器的作用，通过二乙炔聚合诱导的孔形成来响应紫外线辐射，从而引发酶促反应。证实了在外部隔室中荧光β-半乳糖苷酶底物的控制释放和水解，其中反应速率可以通过改变紫外线照射时间来调节。