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NIR upconversion characteristics of carbon dots for selective detection of glutathione†
New Journal of Chemistry ( IF 3.3 ) Pub Date : 2018-03-15 00:00:00 , DOI: 10.1039/c8nj00567b
Satyabrat Gogoi 1, 2, 3, 4, 5 , Raju Khan 1, 2, 3, 4, 5
Affiliation  

In the current study, we report the near infrared (NIR) upconversion (in the range of 850–950 nm) properties of carbon nanoparticles and their utility as a fluorescence probe for selective and sensitive detection of glutathione (GSH). The fluorescence probe comprises of carbon dots (CDs), which possess the capacity to absorb NIR photons (@930 nm) with consequent emission in the visible region (@520–540 nm). CDs, in the presence of Cu2+, suffer severe quenching of its photoluminescence behavior. As a result, the upconversion green emission at 530 nm gets hindered. However, in the presence of GSH, anti-Stoke emission is reinstated courtesy of prominent complexation between GSH and Cu2+. Correlation between the restored upconversion intensity and GSH concentration shows a linear agreement effective for a wide range (0.5–100 μM) with the limit of detection of 0.35 μM. The developed sensing platform also possesses high selectivity in the presence of other amino acids and thiols. Detection of GSH was further carried out in diluted human serum, which revealed a high percentage of recovery. Thus, this study demonstrates the potential of CDs as a cheap, effective and highly selective NIR upconversion based fluorescence probe for the detection of GSH. Such nanoparticles with stable emission could be utilized as the alternative to conventional upconversion organic dyes and semiconductor quantum dots.

中文翻译:

用于选择性检测谷胱甘肽的碳点的近红外上转换特性

在当前的研究中,我们报告了碳纳米颗粒的近红外(NIR)上转换(在850-950 nm范围内)特性,以及它们作为选择性和灵敏检测谷胱甘肽(GSH)的荧光探针的效用。荧光探针由碳点(CD)组成,这些碳点具有吸收NIR光子(@ 930 nm)的能力,并因此在可见光区域(@ 520-540 nm)发射。在Cu 2+存在下,CD遭受其光致发光行为的严重淬灭。结果,阻碍了在530nm的上转换绿色发射。然而,在存在谷胱甘肽的情况下,由于谷胱甘肽和铜2+之间显着的络合,恢复了抗斯托克斯发射。恢复的上转换强度与GSH浓度之间的相关性显示线性协议有效,适用范围广(0.5–100μM),检出限为0.35μM。在存在其他氨基酸和硫醇的情况下,开发的传感平台还具有很高的选择性。在稀释的人血清中进一步进行了GSH的检测,结果显示回收率很高。因此,本研究证明了CDs作为检测GSH的廉价,有效和高度选择性的基于NIR上转换的荧光探针的潜力。具有稳定发射的这种纳米颗粒可以用作常规上转换有机染料和半导体量子点的替代物。
更新日期:2018-03-15
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