Surface enhanced Raman scattering (SERS) generates molecularly specific fingerprints of analytes and when the experimental conditions are carefully controlled this is highly quantitative. This review critiques the development of quantitative SERS from simple univariate assessment of single vibrational modes to multivariate analysis of the whole spectrum for improved quantification. SERS has also been developed for direct multiplex detection and quantification of multiple analytes and this is also discussed, as is the need for LC-SERS for analyte separation should multivariate chemometric approaches fail to effect quantification. Finally, to effect absolute quantification with SERS, the concepts of isotopologues is introduced along with the standard addition method (SAM) and suitable examples that have been developed and exploited these techniques are presented. We believe that SERS will be routinely used for quantitative analysis and it is only a matter of time before this technique translates from the laboratory to the clinical environment.

表面增强拉曼散射（SERS）会生成分析物的分子特异性指纹图谱，当仔细控制实验条件时，这是高度定量的。这篇评论批评了定量SERS的发展，从简单的单振动模态单变量评估到整个光谱的多变量分析以改进量化。还开发了SERS用于多种分析物的直接多重检测和定量，并且也讨论了这一问题，如果多变量化学计量学方法无法实现定量分析，则需要LC-SERS进行分析物分离。最后，要使用SERS进行绝对定量，介绍了同位素同位素的概念以及标准加成方法（SAM），并介绍了已开发并利用这些技术的合适示例。我们相信，SERS将常规用于定量分析，并且该技术从实验室转变为临床环境只是时间问题。