The structural proteins of baculovirus are well studied, but the interactions between them remain unclear. In order to reveal protein-protein interactions among viral structural proteins and their associated proteins of the budded virus of Bombyx mori nucleopolyhedrovirus (BmNPV), the yeast two hybrid (Y2H) system was used to evaluate the interactions of 27 viral genes products. Fifty-seven interactions were identified with 51 binary interactions and 6 self-associations. Among them, 10 interactions were further confirmed by co-immunoprecipitation assays. Five interaction networks were formed based on the direct-cross Y2H assays. VP39, 38 K, and FP were identified to interact with most of the viral proteins, and may form major structural elements of the viral architecture. In addition, each envelope protein was detected to interact with more than one capsid protein. These results suggest how viral structural and structural associated proteins may assemble to form a complete virus through interacting with each other.

杆状病毒的结构蛋白已得到很好的研究，但它们之间的相互作用仍不清楚。为了揭示家蚕发芽病毒的病毒结构蛋白及其相关蛋白之间的蛋白-蛋白相互作用酵母两杂种（Y2H）系统核多角体病毒（BmNPV）用于评估27种病毒基因产物的相互作用。通过51种二元相互作用和6种自我关联，鉴定出57种相互作用。其中，通过免疫共沉淀分析进一步证实了10种相互作用。基于直接交叉Y2H分析，形成了五个相互作用网络。VP39、38 K和FP被确定与大多数病毒蛋白相互作用，并可能形成病毒结构的主要结构元件。另外，检测到每种包膜蛋白与一种以上的衣壳蛋白相互作用。这些结果表明病毒结构和结构相关蛋白如何通过相互相互作用组装成完整的病毒。