Here we describe a novel (−)ssRNA mycovirus, Fusarium graminearum negative-stranded RNA virus 1 (FgNSRV-1), isolated from Fusarium graminearum strain HN1. The genome of FgNSRV-1 is 9072 nucleotides in length, with five discontinuous but linear ORFs (ORF I-V). Phylogenetic analysis based on entire L polymerase sequences indicated that FgNSRV-1 is related to the (−)ssRNA mycovirus Sclerotinia sclerotiorum negative-stranded RNA virus 1 (SsNSRV-1), and other mycoviruses. Our data suggest that FgNSRV-1 can be classified into the family Mymonaviridae, order Mononegavirales. Putative enveloped virion-like structures with filamentous morphology similar to SsNSRV-1 were observed in virion preparation samples. The L proteins of FgNSRV-1, and other fungal mononegaviruses, were found to be related to L protein-like sequences in some fungal genome, supporting the hypothesis that there is coevolution occurring between mycoviruses and fungi. Besides, clearing the virus from the infected host fungus resulted in no discernable phenotypic change.

在这里，我们描述了一种新的（-）ssRNA分支病毒，禾谷镰孢负链RNA病毒1（FgNSRV-1），从禾谷镰孢HN1株中分离出来。FgNSRV-1的基因组长度为9072个核苷酸，具有五个不连续但线性的ORF（ORF IV）。基于完整的L聚合酶序列的系统进化分析表明，FgNSRV-1与（-）ssRNA支原体核盘菌核盘菌负链RNA病毒1（SsNSRV-1）和其他支原体病毒有关。我们的数据表明FgNSRV-1可分为家庭Mymonaviridae，为了Mononegavirales目。在病毒体制备样品中观察到假定的包膜病毒体样结构，其丝状形态类似于SsNSRV-1。发现FgNSRV-1和其他真菌单负病毒的L蛋白与某些真菌基因组中的L蛋白样序列有关，这支持了在分枝杆菌病毒和真菌之间发生共进化的假说。此外，从感染的宿主真菌中清除病毒不会导致明显的表型变化。