The physiological effects of the Stevia-derived compounds, rebaudioside A, stevioside and steviol have been the focus of several studies due to their use as sweeteners in food. Despite that, little is known about their potential food-drug interactions. In the present study, IPEC-J2 cells and primary hepatocytes were used to investigate the effect of rebaudioside A, stevioside and steviol on cytochrome p450 (CYP) mRNA expression. Moreover, hepatic microsomes were used to investigate direct interactions between the compounds and specific CYP activity. In IPEC-J2 no changes in mRNA expression of CYP1A1 or CYP3A29 were observed with the Stevia-derived compounds. In primary hepatocytes all three tested compounds induced a significant increase in CYP3A29 expression. The tested compounds had no direct effect on specific CYP activity. In conclusion, rebaudioside A, stevioside and steviol induce only minor or no changes to the CYP expression and activity, and are not likely to cause food-drug interactions.

甜叶菊衍生的化合物，莱鲍迪苷A，甜菊糖苷和甜菊醇的生理作用由于在食品中用作甜味剂而成为许多研究的重点。尽管如此，对其潜在的食品-药物相互作用知之甚少。在本研究中，使用IPEC-J2细胞和原代肝细胞来研究莱鲍迪苷A，甜菊糖苷和甜菊醇对细胞色素p450（CYP）mRNA表达的影响。此外，肝微粒体用于研究化合物与特定CYP活性之间的直接相互作用。在IPEC-J2中，用甜菊衍生的化合物未观察到CYP1A1或CYP3A29 mRNA表达的变化。在原代肝细胞中，所有三种测试化合物均诱导CYP3A29表达显着增加。所测试的化合物对特定的CYP活性没有直接影响。综上所述，