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The dawning era of oral thin films for nutraceutical delivery: From laboratory to clinic Biotechnol. Adv. (IF 16.0) Pub Date : 2024-04-12 Ruchika, Nabab Khan, Shagun Sanjivv Dogra, Ankit Saneja
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Microbial production of sulfur-containing amino acids using metabolically engineered Escherichia coli Biotechnol. Adv. (IF 16.0) Pub Date : 2024-04-07 Lijuan Wang, Yingying Guo, Yizhou Shen, Kun Yang, Xue Cai, Bo Zhang, Zhiqiang Liu, Yuguo Zheng
L-Cysteine and L-methionine, as the only two sulfur-containing amino acids among the canonical 20 amino acids, possess distinct characteristics and find wide-ranging industrial applications. The use of different organisms for fermentative production of L-cysteine and L-methionine is gaining increasing attention, with being extensively studied as the preferred strain. This preference is due to its ability
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Recent advances in design and application of synthetic membraneless organelles Biotechnol. Adv. (IF 16.0) Pub Date : 2024-04-06 Li Wan, Yingying Zhu, Wenli Zhang, Wanmeng Mu
Membraneless organelles (MLOs) formed by liquid-liquid phase separation (LLPS) have been extensively studied due to their spatiotemporal control of biochemical and cellular processes in living cells. These findings have provided valuable insights into the physicochemical principles underlying the formation and functionalization of biomolecular condensates, which paves the way for the development of
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Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids Biotechnol. Adv. (IF 16.0) Pub Date : 2024-04-06 Zi-Xu Zhang, Ying-Shuang Xu, Zi-Jia Li, Lu-Wei Xu, Wang Ma, Ying-Feng Li, Dong-Sheng Guo, Xiao-Man Sun, He Huang
are marine microorganisms known for their fast growth and ability to store lipids, making them useful for producing polyunsaturated fatty acids (PUFAs), biodiesel, squalene, and carotenoids. However, the high cost of production, mainly due to expensive fermentation components, limits their wider use. A significant challenge in this context is the need to balance production costs with the value of the
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Systematic review on carrageenolytic enzymes: From metabolic pathways to applications in biotechnology Biotechnol. Adv. (IF 16.0) Pub Date : 2024-04-04 Chengcheng Jiang, Yuqi Ma, Wei Wang, Jingjing Sun, Jianhua Hao, Xiangzhao Mao
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Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications Biotechnol. Adv. (IF 16.0) Pub Date : 2024-04-03 Shi-Peng Zhou, Ya-Ping Xue, Yu-Guo Zheng
Nitrilases represent a distinct class of enzymes that play a pivotal role in catalyzing the hydrolysis of nitrile compounds, leading to the formation of corresponding carboxylic acids. These enzymatic entities have garnered significant attention across a spectrum of industries, encompassing pharmaceuticals, agrochemicals, and fine chemicals. Moreover, their significance has been accentuated by mounting
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Inteins: A Swiss army knife for synthetic biology Biotechnol. Adv. (IF 16.0) Pub Date : 2024-03-27 Stanislav Anastassov, Maurice Filo, Mustafa Khammash
Inteins are proteins found in nature that execute protein splicing. Among them, split inteins stand out for their versatility and adaptability, presenting creative solutions for addressing intricate challenges in various biological applications. Their exquisite attributes, including compactness, reliability, orthogonality, low toxicity, and irreversibility, make them of interest to various fields including
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Expanding the horizon of transient CAR T therapeutics using virus-free technology Biotechnol. Adv. (IF 16.0) Pub Date : 2024-03-26 Lucia Enriquez-Rodriguez, Noha Attia, Idoia Gallego, Mohamed Mashal, Iván Maldonado, Gustavo Puras, José Luis Pedraz
The extraordinary success that chimeric antigen receptor (CAR) T cell therapies have shown over the years on fighting hematological malignancies is evidenced by the six FDA-approved products present on the market. CAR T treatments have forever changed the way we understand cellular immunotherapies, as current research in the topic is expanding even outside the field of cancer with very promising results
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The production, recovery, and valorization of polyhydroxybutyrate (PHB) based on circular bioeconomy Biotechnol. Adv. (IF 16.0) Pub Date : 2024-03-26 Jianfei Wang, Jiaqi Huang, Shijie Liu
As an energy-storage substance of microorganisms, polyhydroxybutyrate (PHB) is a promising alternative to petrochemical polymers. Under appropriate fermentation conditions, PHB-producing strains with metabolic diversity can efficiently synthesize PHB using various carbon sources. Carbon-rich wastes may serve as alternatives to pure sugar substrates to reduce the cost of PHB production. Genetic engineering
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Rational construction of synthetic consortia: Key considerations and model-based methods for guiding the development of a novel biosynthesis platform Biotechnol. Adv. (IF 16.0) Pub Date : 2024-03-24 Yu Liu, Boyuan Xue, Hao Liu, Shaojie Wang, Haijia Su
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Biocatalysis of CO2 and CH4: Key enzymes and challenges Biotechnol. Adv. (IF 16.0) Pub Date : 2024-03-23 Aipeng Li, Xupeng Cao, Rongzhan Fu, Shuqi Guo, Qiang Fei
Mitigating greenhouse gas emissions is a critical challenge for promoting global sustainability. The utilization of CO and CH as substrates for the production of valuable products offers a promising avenue for establishing an eco-friendly economy. Biocatalysis, a sustainable process utilizing enzymes to facilitate biochemical reactions, plays a significant role in upcycling greenhouse gases. This review
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Mobile genetic element-based gene editing and genome engineering: Recent advances and applications Biotechnol. Adv. (IF 16.0) Pub Date : 2024-03-22 Jaeseong Hwang, Dae-Yeol Ye, Gyoo Yeol Jung, Sungho Jang
Genome engineering has revolutionized several scientific fields, ranging from biochemistry and fundamental research to therapeutic uses and crop development. Diverse engineering toolkits have been developed and used to effectively modify the genome sequences of organisms. However, there is a lack of extensive reviews on genome engineering technologies based on mobile genetic elements (MGEs), which
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Harnessing recalcitrant lignocellulosic biomass for enhanced biohydrogen production: Recent advances, challenges, and future perspective Biotechnol. Adv. (IF 16.0) Pub Date : 2024-03-21 Sameh S. Ali, Rania Al-Tohamy, Tamer Elsamahy, Jianzhong Sun
Biohydrogen (Bio-H) is widely recognized as a sustainable and environmentally friendly energy source, devoid of any detrimental impact on the environment. Lignocellulosic biomass (LB) is a readily accessible and plentiful source material that can be effectively employed as a cost-effective and sustainable substrate for Bio-H production. Despite the numerous challenges, the ongoing progress in LB pretreatment
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Diverse models of cavity engineering in enzyme modification: Creation, filling, and reshaping Biotechnol. Adv. (IF 16.0) Pub Date : 2024-03-20 Zehua Zhang, Yongchao Cai, Nan Zheng, Yu Deng, Ling Gao, Qiong Wang, Xiaole Xia
Most enzyme modification strategies focus on designing the active sites or their surrounding structures. Interestingly, a large portion of the enzymes (60%) feature active sites located within spacious cavities. Despite recent discoveries, cavity-mediated enzyme engineering remains crucial for enhancing enzyme properties and unraveling folding-unfolding mechanisms. Cavity engineering influences enzyme
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Engineering aspects of lipid-based delivery systems: In vivo gene delivery, safety criteria, and translation strategies Biotechnol. Adv. (IF 16.0) Pub Date : 2024-03-20 Ismail Eş, Aneesh Thakur, Amin Mousavi Khaneghah, Camilla Foged, Lucimara Gaziola de la Torre
Defects in the genome cause genetic diseases and can be treated with gene therapy. Due to the limitations encountered in gene delivery, lipid-based supramolecular colloidal materials have emerged as promising gene carrier systems. In their non-functionalized form, lipid nanoparticles often demonstrate lower transgene expression efficiency, leading to suboptimal therapeutic outcomes, specifically through
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A domain swapping strategy to create modular transcriptional regulators for novel topology in genetic network Biotechnol. Adv. (IF 16.0) Pub Date : 2024-03-19 Clement T.Y. Chan, Vincenzo Kennedy, Sahaj Kinshuk
Transcriptional regulators generate connections between biological signals and genetic outputs. They are used robustly for sensing input signals in building genetic circuits. However, each regulator can only generate a fixed connection, which generates constraints in linking multiple signals for more complex processes. Recent studies discovered that a domain swapping strategy can be applied to various
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Transcription factor-based biosensor: A molecular-guided approach for advanced biofuel synthesis Biotechnol. Adv. (IF 16.0) Pub Date : 2024-03-18 Minrui Lu, Yuanyuan Sha, Vinod Kumar, Zhaoxian Xu, Rui Zhai, Mingjie Jin
As a sustainable and renewable alternative to petroleum fuels, advanced biofuels shoulder the responsibility of energy saving, emission reduction and environmental protection. Traditional engineering of cell factories for production of advanced biofuels lacks efficient high-throughput screening tools and regulating systems, impeding the improvement of cellular productivity and yield. Transcription
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Multifaced application of AFEX-pretreated biomass in producing second-generation biofuels, ruminant animal feed, and value-added bioproducts Biotechnol. Adv. (IF 16.0) Pub Date : 2024-03-16 Maedeh Mohammadi, Mahsa Alian, Bruce Dale, Bryan Ubanwa, Venkatesh Balan
Lignocellulosic biomass holds a crucial position in the prospective bio-based economy, serving as a sustainable and renewable source for a variety of bio-based products. These products play a vital role in displacing fossil fuels and contributing to environmental well-being. However, the inherent recalcitrance of biomass poses a significant obstacle to the efficient access of sugar polymers. Consequently
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Recent advances in phyto-combined remediation of heavy metal pollution in soil Biotechnol. Adv. (IF 16.0) Pub Date : 2024-03-07 Shaoxiong Deng, Xuan Zhang, Yonghua Zhu, Rui Zhuo
The global industrialization and modernization have witnessed a rapid progress made in agricultural production, along with the issue of soil heavy metal (HM) pollution, which has posed severe threats to soil quality, crop yield, and human health. Phytoremediation, as an alternative to physical and chemical methods, offers a more cost-effective, eco-friendly, and aesthetically appealing means for in-situ
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Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions Biotechnol. Adv. (IF 16.0) Pub Date : 2024-03-07 Chengmei Yin, Xiangyao Chen, Haiyang Zhang, Yong Xue, Hao Dong, Xiangzhao Mao
Non-homogeneous enzyme-catalyzed systems are more widely used than homogeneous systems. Distinguished from the conventional biphasic approach, Pickering emulsion stabilized by ultrafine solid particles opens up an innovative platform for biocatalysis. Their vast specific surface area significantly enhances enzyme-substrate interactions, dramatically increasing catalytic efficiency. This review comprehensively
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Next generation probiotics: Engineering live biotherapeutics Biotechnol. Adv. (IF 16.0) Pub Date : 2024-03-02 Sanjeeva Kumar Murali, Thomas J. Mansell
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The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future Biotechnol. Adv. (IF 16.0) Pub Date : 2024-02-27 Jianlong Wang, Xuan Guo
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New opportunities and perspectives on biosynthesis and bioactivities of secondary metabolites from Aloe vera Biotechnol. Adv. (IF 16.0) Pub Date : 2024-02-21 Mrudulakumari Vasudevan Ushasree, Qi Jia, Seon Gil Do, Eun Yeol Lee
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Progress in cyclodextrins as important molecules regulating catalytic processes of glycoside hydrolases Biotechnol. Adv. (IF 16.0) Pub Date : 2024-02-19 Xiaoxiao Li, Zhengyu Jin, Yuxiang Bai, Birte Svensson
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Emerging advances in glycoengineering of carbohydrates/glycans and their industrial applications Biotechnol. Adv. (IF 16.0) Pub Date : 2024-02-13 Brahma N. Singh, Meisam Tabatabaei, Abhay Pandit, Lothar Elling, Vijai Kumar Gupta
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CRISPR-Cas systems of lactic acid bacteria and applications in food science Biotechnol. Adv. (IF 16.0) Pub Date : 2024-02-11 Yanhua Cui, Xiaojun Qu
CRISPR-Cas (Clustered regularly interspaced short palindromic repeats-CRISPR associated proteins) systems are widely distributed in lactic acid bacteria (LAB), contributing to their RNA-mediated adaptive defense immunity. The CRISPR-Cas-based genetic tools have exhibited powerful capability. It has been highly utilized in different organisms, accelerating the development of life science. The review
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The thin line between monooxygenases and peroxygenases. P450s, UPOs, MMOs, and LPMOs: A brick to bridge fields of expertise Biotechnol. Adv. (IF 16.0) Pub Date : 2024-02-08 Davide Decembrino, David Cannella
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Decoding cellular mechanism of recombinant adeno-associated virus (rAAV) and engineering host-cell factories toward intensified viral vector manufacturing Biotechnol. Adv. (IF 16.0) Pub Date : 2024-02-07 Yongdan Wang, Qiang Fu, So Young Park, Yong Suk Lee, Seo-Young Park, Dong-Yup Lee, Seongkyu Yoon
Recombinant adeno-associated virus (rAAV) is one of the prominent gene delivery vehicles that has opened promising opportunities for novel gene therapeutic approaches. However, the current major viral vector production platform, triple transfection in mammalian cells, may not meet the increasing demand. Thus, it is highly required to understand production bottlenecks from the host cell perspective
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Genome-scale metabolic network models for industrial microorganisms metabolic engineering: Current advances and future prospects Biotechnol. Adv. (IF 16.0) Pub Date : 2024-01-26 Zhijin Gong, Jiayao Chen, Xinyu Jiao, Hao Gong, Danzi Pan, Lingli Liu, Yang Zhang, Tianwei Tan
The construction of high-performance microbial cell factories (MCFs) is the centerpiece of biomanufacturing. However, the complex metabolic regulatory network of microorganisms poses great challenges for the efficient design and construction of MCFs. The genome-scale metabolic network models (GSMs) can systematically simulate the metabolic regulation process of microorganisms , providing effective
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Molecular imprinting technology for biomedical applications Biotechnol. Adv. (IF 16.0) Pub Date : 2024-01-22 Julija Sarvutienė, Urte Samukaite-Bubniene, Simonas Ramanavicius, Arunas Ramanavicius
Molecularly imprinted polymers (MIPs), a type of biomimetic material, have attracted considerable interest owing to their cost-effectiveness, good physiochemical stability, favourable specificity and selectivity for target analytes, and widely used for various biological applications. It was demonstrated that MIPs with significant selectivity towards protein-based targets could be applied in medicine
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PHA is not just a bioplastic! Biotechnol. Adv. (IF 16.0) Pub Date : 2024-01-23 Helen Park, Hongtao He, Xu Yan, Xu Liu, Nigel S. Scrutton, Guo-Qiang Chen
Polyhydroxyalkanoates (PHA) have evolved into versatile biopolymers, transcending their origins as mere bioplastics. This extensive review delves into the multifaceted landscape of PHA applications, shedding light on the diverse industries that have harnessed their potential. PHA has proven to be an invaluable eco-conscious option for packaging materials, finding use in films foams, paper coatings
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Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review Biotechnol. Adv. (IF 16.0) Pub Date : 2024-01-09 Jinsong Liang, Ru Zhang, Jianning Chang, Le Chen, Mohammad Nabi, Haibo Zhang, Guangming Zhang, Panyue Zhang
The rumen of ruminants is a natural anaerobic fermentation system that efficiently degrades lignocellulosic biomass and mainly depends on synergistic interactions between multiple microbes and their secreted enzymes. Ruminal microbes have been employed as biomass waste converters and are receiving increasing attention because of their degradation performance. To explore the application of ruminal microbes
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Passive microfluidic devices for cell separation Biotechnol. Adv. (IF 16.0) Pub Date : 2024-01-12 Tianlong Zhang, Dino Di Carlo, Chwee Teck Lim, Tianyuan Zhou, Guizhong Tian, Tao Tang, Amy Q. Shen, Weihua Li, Ming Li, Yang Yang, Keisuke Goda, Ruopeng Yan, Cheng Lei, Yoichiroh Hosokawa, Yaxiaer Yalikun
The separation of specific cell populations is instrumental in gaining insights into cellular processes, elucidating disease mechanisms, and advancing applications in tissue engineering, regenerative medicine, diagnostics, and cell therapies. Microfluidic methods for cell separation have propelled the field forward, benefitting from miniaturization, advanced fabrication technologies, a profound understanding
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Resource allocation in mammalian systems Biotechnol. Adv. (IF 16.0) Pub Date : 2024-01-11 Hratch M. Baghdassarian, Nathan E. Lewis
Cells execute biological functions to support phenotypes such as growth, migration, and secretion. Complementarily, each function of a cell has resource costs that constrain phenotype. Resource allocation by a cell allows it to manage these costs and optimize their phenotypes. In fact, the management of resource constraints (e.g., nutrient availability, bioenergetic capacity, and macromolecular machinery
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Ionic liquids and deep eutectic solvents for the stabilization of biopharmaceuticals: A review Biotechnol. Adv. (IF 16.0) Pub Date : 2024-01-08 Nathalia Vieira Porphirio Veríssimo, Cassamo Usemane Mussagy, Heitor Buzetti Simões Bento, Jorge Fernando Brandão Pereira, Valéria de Carvalho Santos-Ebinuma
Biopharmaceuticals have allowed the control of previously untreatable diseases. However, their low solubility and stability still hinder their application, transport, and storage. Hence, researchers have applied different compounds to preserve and enhance the delivery of biopharmaceuticals, such as ionic liquids (ILs) and deep eutectic solvents (DESs). Although the biopharmaceutical industry can employ
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A guide to the use of bioassays in exploration of natural resources Biotechnol. Adv. (IF 16.0) Pub Date : 2024-01-05 Jerica Sabotič, Engin Bayram, David Ezra, Susana P. Gaudêncio, Berat Z. Haznedaroğlu, Nika Janež, Leila Ktari, Anna Luganini, Manolis Mandalakis, Ivo Safarik, Dina Simes, Evita Strode, Anna Toruńska-Sitarz, Despoina Varamogianni-Mamatsi, Giovanna Cristina Varese, Marlen I. Vasquez
Bioassays are the main tool to decipher bioactivities from natural resources thus their selection and quality are critical for optimal bioprospecting. They are used both in the early stages of compounds isolation/purification/identification, and in later stages to evaluate their safety and efficacy. In this review, we provide a comprehensive overview of the most common bioassays used in the discovery
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Strategies for the biological production of ectoine by using different chassis strains Biotechnol. Adv. (IF 16.0) Pub Date : 2023-12-28 Yifan Feng, Min Qiu, Lei Shao, Yujia Jiang, Wenming Zhang, Wankui Jiang, Fengxue Xin, Min Jiang
As an amino acid derivative and a typical compatible solute, ectoine can assist microorganisms in resisting high osmotic pressure. Own to its long-term moisturizing effects, ectoine shows extensive applications in cosmetics, medicine and other fields. With the rapid development of synthetic biology and fermentation engineering, many biological strategies have been developed to improve the ectoine production
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Survival strategies of Bacillus spp. in saline soils: Key factors to promote plant growth and health Biotechnol. Adv. (IF 16.0) Pub Date : 2023-12-19 María F. Valencia-Marin, Salvador Chávez-Avila, Paulina Guzmán-Guzmán, Ma del Carmen Orozco-Mosqueda, Sergio de los Santos-Villalobos, Bernard R. Glick, Gustavo Santoyo
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Proteases immobilized on nanomaterials for biocatalytic, environmental and biomedical applications: Advantages and drawbacks Biotechnol. Adv. (IF 16.0) Pub Date : 2023-12-21 Muhammad Bilal, Sarmad Ahmad Qamar, Diego Carballares, Ángel Berenguer-Murcia, Roberto Fernandez-Lafuente
Proteases have gained significant scientific and industrial interest due to their unique biocatalytic characteristics and broad-spectrum applications in different industries. The development of robust nanobiocatalytic systems by attaching proteases onto various nanostructured materials as fascinating and novel nanocarriers has demonstrated exceptional biocatalytic performance, substantial stability
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Bacillus genus industrial applications and innovation: First steps towards a circular bioeconomy Biotechnol. Adv. (IF 16.0) Pub Date : 2023-12-14 Leonardo Wedderhoff Herrmann, Luiz Alberto Junior Letti, Rafaela de Oliveira Penha, Vanete Thomaz Soccol, Cristine Rodrigues, Carlos Ricardo Soccol
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Utilization of gene manipulation system for advancing the biotechnological potential of halophiles: A review Biotechnol. Adv. (IF 16.0) Pub Date : 2023-12-13 Tong Xu, Ruchira Mitra, Dan Tan, Zhengjun Li, Cheng Zhou, Tao Chen, Zhengwei Xie, Jing Han
Halophiles are salt-loving microorganisms known to have their natural resistance against media contamination even when cultivated in nonsterile and continuous bioprocess system, thus acting as promising cell factories for Next Generation of Industrial Biotechnology (NGIB). NGIB — a successor to the traditional industrial biotechnology, is a more sustainable and efficient bioprocess technology while
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Pioneering DNA assembling techniques and their applications in eukaryotic microalgae Biotechnol. Adv. (IF 16.0) Pub Date : 2023-12-13 Li Guo, Guanpin Yang
Assembling DNA fragments is a fundamental manipulation of cloning microalgal genes and carrying out microalgal synthetic biological studies. From the earliest DNA recombination to current trait and metabolic pathway engineering, we are always accompanied by homology-based DNA assembling. The improvement and modification of pioneering DNA assembling techniques and the combinational applications of the
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Bioinspired nanoplatforms for human-machine interfaces: Recent progress in materials and device applications Biotechnol. Adv. (IF 16.0) Pub Date : 2023-12-06 Pasha W. Sayyad, Sang-Joon Park, Tae-Jun Ha
The panoramic characteristics of human–machine interfaces (HMIs) have prompted the needs to update the biotechnology community with the recent trends, developments, and future research direction toward next-generation bioelectronics. Bioinspired materials are promising for integrating various bioelectronic devices to realize HMIs. With the advancement of scientific biotechnology, state-of-the-art bioelectronic
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Metal-organic frameworks for enzyme immobilization and nanozymes: A laccase-focused review Biotechnol. Adv. (IF 16.0) Pub Date : 2023-12-10 Lynette Alvarado-Ramírez, Gerson Machorro-García, Andrea López-Legarrea, Dulce Trejo-Ayala, Magdalena de Jesús Rostro-Alanis, Manuel Sánchez-Sánchez, Rosa M. Blanco, José Rodríguez-Rodríguez, Roberto Parra-Saldívar
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Discovering the secondary metabolic potential of Saccharothrix Biotechnol. Adv. (IF 16.0) Pub Date : 2023-12-03 Bin Wei, Xian Luo, Zhen-Yi Zhou, Gang-Ao Hu, Lei Li, Hou-Wen Lin, Hong Wang
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Universal and unique strategies for the production of polyunsaturated fatty acids in industrial oleaginous microorganisms Biotechnol. Adv. (IF 16.0) Pub Date : 2023-12-02 Chun-Xiao Yan, Ying Zhang, Wen-Qian Yang, Wang Ma, Xiao-Man Sun, He Huang
Polyunsaturated fatty acids (PUFAs), especially docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and arachidonic acid (ARA), are beneficial for reducing blood cholesterol and enhancing memory. Traditional PUFA production relies on extraction from plants and animals, which is unsustainable. Thus, using microorganisms as lipid-producing factories holds promise as an alternative way for PUFA production
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Recombinant production of antimicrobial peptides in plants Biotechnol. Adv. (IF 16.0) Pub Date : 2023-12-01 Farhad Nazarian-Firouzabadi, Marcelo Der Torossian Torres, Cesar de la Fuente-Nunez
Classical plant breeding methods are limited in their ability to confer disease resistance on plants. However, in recent years, advancements in molecular breeding and biotechnological have provided new approaches to overcome these limitations and protect plants from disease. Antimicrobial peptides (AMPs) constitute promising agents that may be able to protect against infectious agents. Recently, peptides
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Artificial carbon assimilation: From unnatural reactions and pathways to synthetic autotrophic systems Biotechnol. Adv. (IF 16.0) Pub Date : 2023-11-25 Xue Yang, Yanfei Zhang, Guoping Zhao
Synthetic biology is being increasingly used to establish novel carbon assimilation pathways and artificial autotrophic strains that can be used in low-carbon biomanufacturing. Currently, artificial pathway design has made significant progress from advocacy to practice within a relatively short span of just over ten years. However, there is still huge scope for exploration of pathway diversity, operational
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Challenges in developing cell culture media using machine learning Biotechnol. Adv. (IF 16.0) Pub Date : 2023-11-19 Takamasa Hashizume, Bei-Wen Ying
Microbial and mammalian cells are widely used in the food, pharmaceutical, and medical industries. Developing or optimizing culture media is essential to improve cell culture performance as a critical technology in cell culture engineering. Methodologies for media optimization have been developed to a great extent, such as the approaches of one-factor-at-a-time (OFAT) and response surface methodology
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Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens Biotechnol. Adv. (IF 16.0) Pub Date : 2023-11-14 Maddy L. Newby, Joel D. Allen, Max Crispin
A key aspect of successful viral vaccine design is the elicitation of neutralizing antibodies targeting viral attachment and fusion glycoproteins that embellish viral particles. This observation has catalyzed the development of numerous viral glycoprotein mimetics as vaccines. Glycans can dominate the surface of viral glycoproteins and as such, the viral glycome can influence the antigenicity and immunogenicity
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Engineering status of protein for improving microbial cell factories Biotechnol. Adv. (IF 16.0) Pub Date : 2023-11-07 Pei Zhou, Cong Gao, Wei Song, Wanqing Wei, Jing Wu, Liming Liu, Xiulai Chen
With the development of metabolic engineering and synthetic biology, microbial cell factories (MCFs) have provided an efficient and sustainable method to synthesize a series of chemicals from renewable feedstocks. However, the efficiency of MCFs is usually limited by the inappropriate status of protein. Thus, engineering status of protein is essential to achieve efficient bioproduction with high titer
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Production and characterization of starch-lignin based materials: A review Biotechnol. Adv. (IF 16.0) Pub Date : 2023-11-11 Jonathan A. Diaz-Baca, Pedram Fatehi
In their pristine state, starch and lignin are abundant and inexpensive natural polymers frequently considered green alternatives to oil-based and synthetic polymers. Despite their availability and owing to their physicochemical properties; starch and lignin are not often utilized in their pristine forms for high-performance applications. Generally, chemical and physical modifications transform them
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Abiotic stress as a dynamic strategy for enhancing high value phytochemicals in microalgae: Critical insights, challenges and future prospects Biotechnol. Adv. (IF 16.0) Pub Date : 2023-11-07 Uganeeswary Suparmaniam, Man Kee Lam, Jun Wei Lim, Inn Shi Tan, Bridgid Lai Fui Chin, Siew Hoong Shuit, Steven Lim, Yean Ling Pang, Peck Loo Kiew
Microalgae showcase an extraordinary capacity for synthesizing high-value phytochemicals (HVPCs), offering substantial potential for diverse applications across various industries. Emerging research suggests that subjecting microalgae to abiotic stress during cultivation and the harvesting stages can further enhance the accumulation of valuable metabolites within their cells, including carotenoids
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Prospects and hazards of silica nanoparticles: Biological impacts and implicated mechanisms Biotechnol. Adv. (IF 16.0) Pub Date : 2023-11-03 Ruiyang Ding, Yang Li, Yang Yu, Zhiwei Sun, Junchao Duan
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Has the time finally come for green oleochemicals and biodiesel production using large-scale enzyme technologies? Current status and new developments Biotechnol. Adv. (IF 16.0) Pub Date : 2023-10-30 Ahmad Mustafa, Shah Faisal, Inas A. Ahmed, Mamoona Munir, Eliane Pereira Cipolatti, Evelin Andrade Manoel, Carlo Pastore, Luigi di Bitonto, Dieter Hanelt, Febri Odel Nitbani, Zeinhom M. El-Bahy, Abrar Inayat, Tamer M.M. Abdellatief, Konstantza Tonova, Awais Bokhari, Abdelfatah Abomohra
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Unleashing the capacity of Rhodococcus for converting lignin into lipids Biotechnol. Adv. (IF 16.0) Pub Date : 2023-10-31 Zhi-Min Zhao, Zhi-Hua Liu, Tongtong Zhang, Rongqian Meng, Zhiqun Gong, Yibing Li, Jing Hu, Arthur J. Ragauskas, Bing-Zhi Li, Ying-Jin Yuan
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Cell wall polysaccharides of streptococci: A genetic and structural perspective Biotechnol. Adv. (IF 16.0) Pub Date : 2023-10-31 Zoe Kampff, Douwe van Sinderen, Jennifer Mahony
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Next-generation feedstocks methanol and ethylene glycol and their potential in industrial biotechnology Biotechnol. Adv. (IF 16.0) Pub Date : 2023-10-31 Nils Wagner, Linxuan Wen, Cláudio J.R. Frazão, Thomas Walther
Microbial fermentation processes are expected to play an important role in reducing dependence on fossil-based raw materials for the production of everyday chemicals. In order to meet the growing demand for biotechnological products in the future, alternative carbon sources that do not compete with human nutrition must be exploited. The chemical conversion of the industrially emitted greenhouse gas
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Bacillus sp. as a microbial cell factory: Advancements and future prospects Biotechnol. Adv. (IF 16.0) Pub Date : 2023-10-28 Jinyi Qian, Yuzhou Wang, Zijian Hu, Tianqiong Shi, Yuetong Wang, Chao Ye, He Huang
Bacillus sp. is one of the most distinctive gram-positive bacteria, able to grow efficiently using cheap carbon sources and secrete a variety of useful substances, which are widely used in food, pharmaceutical, agricultural and environmental industries. At the same time, Bacillus sp. is also recognized as a safe genus with a relatively clear genetic background, which is conducive to the industrial