Four various biosensors based on flow enzymatic mini‐reactors containing one of the two different mesoporous silica powders (SBA‐15 or MCM‐41) modified by one of the two enzymes (laccase or tyrosinase) were used for the determination of L‐DOPA, dopamine, noradrenaline, and adrenaline. Dependences of peak currents on detection potentials, flow rates, injected volumes, reactor volumes, and analyte concentrations were studied and optimized. The silica powder of MCM‐41 with covalently bonded laccase (Lac) was found to be the best filling for the detection of catecholamines. Relative current response of such biosensor was: 100 % for dopamine, 32.1 % for L‐DOPA, 26.2 % for noradrenaline, and 0.71 % for adrenaline. Statistical evaluation of parallel determinations of studied analytes with this biosensor proved its good accuracy (RSD=1.82–5.18 %) and sensitivity (5.46–15.5 μmol L⁻¹). Life time of the studied biosensors is several months. The practical applicability of Lac‐MCM41 biosensor was successfully verified by the determination of dopamine and noradrenaline in medical solutions for infusions.

中文翻译：

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流动系统中酶生物传感器安培测定邻苯二酚

四种基于流式酶微型反应器的生物传感器包含两种酶（漆酶或酪氨酸酶）之一修饰的两种不同介孔二氧化硅粉末（SBA-15或MCM-41）之一，用于测定L-DOPA，多巴胺，去甲肾上腺素和肾上腺素。研究和优化了峰值电流对检测电位，流速，进样量，反应器体积和分析物浓度的依赖性。发现MCM-41的二氧化硅粉末与共价键结合的漆酶（Lac）是检测儿茶酚胺的最佳填充物。这种生物传感器的相对电流响应为：多巴胺为100％，L-DOPA为32.1％，去甲肾上腺素为26.2％，肾上腺素为0.71％。使用该生物传感器对研究的分析物进行平行测定的统计评估证明了其良好的准确性（RSD = 1.82–5.18％）和灵敏度（5.46–15.5μmolL ^-1）。被研究的生物传感器的使用寿命为几个月。Lac-MCM41生物传感器的实际适用性已通过确定输液医用溶液中的多巴胺和去甲肾上腺素而成功验证。