当前位置: X-MOL 学术ACS Nano › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Effect of Cell Sex on Uptake of Nanoparticles: The Overlooked Factor at the Nanobio Interface
ACS Nano ( IF 17.1 ) Pub Date : 2018-03-14 00:00:00 , DOI: 10.1021/acsnano.7b06212
Vahid Serpooshan 1, 2 , Sara Sheibani 3 , Pooja Pushparaj 4 , Michal Wojcik 5 , Albert Y. Jang 6 , Michelle R. Santoso 6 , Joyce H. Jang 7 , Haina Huang 5 , Reihaneh Safavi-Sohi 8 , Niloofar Haghjoo 9 , Hossein Nejadnik 10 , Haniyeh Aghaverdi 11 , Hojatollah Vali 3 , Joseph Matthew Kinsella 4 , John Presley 3 , Ke Xu 5, 12 , Phillip Chung-Ming Yang 6 , Morteza Mahmoudi 6, 11
Affiliation  

Cellular uptake of nanoparticles (NPs) depends on the nature of the nanobio system including the solid nanocomponents (e.g., physicochemical properties of NPs), nanobio interfaces (e.g., protein corona composition), and the cellular characteristics (e.g., cell type). In this study, we document the role of sex in cellular uptake of NPs as an “overlooked” factor in nanobio interface investigations. We demonstrate that cell sex leads to differences in NP uptake between male and female human amniotic stem cells (hAMSCs), with greater uptake by female cells. hAMSCs are one of the earliest sources of somatic stem cells. The experiments were replicated with primary fibroblasts isolated from the salivary gland of adult male and female donors of similar ages, and again the extent of NP uptake was altered by cell sex. However, in contrast to hAMSCs, uptake was greater in male cells. We also found out that female versus male amniotic stem cells exhibited different responses to reprogramming into induced pluripotent stem cells (iPSCs) by the Yamanaka factors. Thus, future studies should consider the effect of sex on the nanobio interactions to optimize clinical translation of NPs and iPSC biology and to help researchers to better design and produce safe and efficient therapeutic sex-specific NPs.

中文翻译:

细胞性别对纳米颗粒吸收的影响:在纳米生物界面被忽视的因素。

的纳米颗粒(NP)的细胞摄取依赖于的性质纳米生物系统包括固体纳米组分(É,NP的物理化学性质),纳米生物界面É,蛋白质电晕组合物),并且蜂窝特征è。,单元格类型)。在这项研究中,我们记录了性别在纳米颗粒细胞摄取中的作用,作为纳米生物界面研究中的“被忽视”因素。我们证明细胞性别导致男性和女性人类羊水干细胞(hAMSCs)之间的NP摄取差异,女性细胞的摄取更大。hAMSC是体干细胞的最早来源之一。用从年龄相似的成年雄性和雌性供体的唾液腺分离出的原代成纤维细胞进行了重复实验,并且再次通过细胞性别改变了NP的吸收程度。但是,与hAMSC相比,雄性细胞的摄取更大。我们还发现,女性男性羊水干细胞对Yamanaka因子重编程为诱导性多能干细胞(iPSC)表现出不同的反应。因此,未来的研究应考虑性别对纳米生物相互作用的影响,以优化NP和iPSC生物学的临床翻译,并帮助研究人员更好地设计和生产安全有效的性别特异性NP。
更新日期:2018-03-14
down
wechat
bug