Enzyme-mimicking catalytic nanoparticles, more commonly known as NanoZymes, have been at the forefront for the development of new sensing platforms for the detection of a range of molecules. Although solution-based NanoZymes have shown promise in glucose detection, the ability to immobilize NanoZymes on highly absorbent surfaces, particularly on free-standing substrates that can be feasibly exposed and removed from the reaction medium, can offer significant benefits for a range of biosensing and catalysis applications. This work, for the first time, shows the ability of Ag nanoparticles embedded within the 3D matrix of a cotton fabric to act as a free-standing peroxidase-mimic NanoZyme for the rapid detection of glucose in complex biological fluids such as urine. The use of cotton fabric as a template not only allows high number of catalytically active sites to participate in the enzyme-mimic catalytic reaction, the absorbent property of the cotton fibres also helps in rapid absorption of biological molecules such as glucose during the sensing event. This, in turn, brings the target molecule of interest in close proximity of the NanoZyme catalyst enabling accurate detection of glucose in urine. Additionally, the ability to extract the free-standing cotton fabric-supported NanoZyme following the reaction overcomes the issue of potential interference from colloidal nanoparticles during the assay. Based on these unique characteristics, nanostructured silver fabrics offer remarkable promise for the detection of glucose and other biomolecules in complex biological and environmental fluids.

模仿酶的催化纳米颗粒（通常称为NanoZymes）在开发用于检测一系列分子的新传感平台方面一直处于前沿。尽管基于溶液的NanoZymes在葡萄糖检测中已显示出希望，但将NanoZymes固定在高吸收性表面上的能力，特别是在可以从反应介质中适当暴露或移出的自支撑底物上，可以为一系列生物传感和生物降解提供显着的好处。催化应用。这项工作首次显示了嵌入棉织物3D基质中的Ag纳米颗粒具有作为独立的过氧化物酶模拟NanoZyme的能力，可用于快速检测复杂生物流体（如尿液）中的葡萄糖。使用棉织物作为模板，不仅允许大量的催化活性位点参与酶模拟的催化反应，而且棉纤维的吸收特性还有助于在感测事件期间快速吸收诸如葡萄糖的生物分子。反过来，这使目标靶分子与NanoZyme催化剂紧密相邻，从而能够准确检测尿液中的葡萄糖。另外，反应后提取自支撑棉织物支撑的NanoZyme的能力克服了测定过程中胶体纳米颗粒潜在干扰的问题。基于这些独特的特性，纳米结构的银纤维织物为检测复杂生物和环境流体中的葡萄糖和其他生物分子提供了广阔的前景。