Recently, fullerene (C₆₀) and its derivatives have attracted tremendous research interest in diverse fields owing to their excellent fluorescence and biological properties. In this work, the carboxyl groups containing fluorescent C₆₀COOH nanoparticles have been prepared via a one-step thiol-ene click reaction for the first time. Then, we would further demonstrate that C₆₀COOH nanoparticles possess excellent water dispersibility, low cytotoxicity, outstanding fluorescence properties and uniform minuscule based on various characterization techniques. On account of these advantages, we further evaluate their performance in cell imaging. Furthermore, the carboxyl groups on the surface of C₆₀COOH nanoparticles could not only improve their water dispersibility, but also be potentially used to conjugate with various biomolecules and drug molecules. Based on these carboxyl groups, we loaded the anticancer agent cisplatin on C₆₀COOH nanoparticles and further explore their potential for controlled drug release. Taken together, we have developed a novel thiol-ene click reaction for preparation of carboxyl groups functionalized fluorescent C₆₀ based nanoparticles, which showed great potential for biological imaging and potential for intracellular controlled drug delivery. More importantly, this strategy developed in this work should also be expanded for fabrication of other fluorescent C₆₀ based nanoparticles through utilizing different thiol-containing compounds and other fluorescent carbon nanoparticles. Therefore, this work will provide some novel ideas for the preparation of various fluorescent carbon nanoparticles for theranostic applications.

近来，富勒烯（C ₆₀）及其衍生物由于其优异的荧光和生物学性质而引起了在各个领域的巨大研究兴趣。在这项工作中，含羧基的荧光C ₆₀ COOH纳米粒子是首次通过一步式硫醇-烯点击反应制备的。然后，基于各种表征技术，我们将进一步证明C ₆₀ COOH纳米粒子具有优异的水分散性，低细胞毒性，出色的荧光性质和均一的微粒。由于这些优点，我们进一步评估了它们在细胞成像中的性能。此外，C ₆₀表面的羧基COOH纳米颗粒不仅可以改善其水分散性，而且还可以潜在地与各种生物分子和药物分子结合。基于这些羧基，我们将抗癌药顺铂负载在C ₆₀ COOH纳米颗粒上，并进一步探索了其可控药物释放的潜力。综上所述，我们已经开发出一种新型的硫醇-烯点击反应，用于制备羧基官能化的基于荧光C ₆₀的纳米粒子，该反应显示出巨大的生物成像潜能和细胞内可控药物传递潜能。更重要的是，这项工作中制定的这一策略也应扩展到制造其他荧光C ₆₀通过利用不同的含硫醇化合物和其他荧光碳纳米颗粒来制备基于纳米的纳米颗粒。因此，这项工作将为制备治疗用的各种荧光碳纳米粒子提供一些新颖的思路。