Calcium ion (Ca²⁺) plays a critical and indispensable role in many physiological and biochemical processes in the human body. In this report, we demonstrate a novel electrochemical method for the determination of the Ca²⁺ level aided by three functional DNA probes and gold nanoparticles (AuNPs). It affords high selectivity in sensing Ca²⁺ over other metal cations, which is due to the adoption of the DNAzyme at the electrode interface with exceptionally high binding ability. This method also integrates recycling formation of DNAzyme and AuNPs-mediated amplification; thus, high sensitivity is promised. Therefore, this work provides a favorable way to probe Ca²⁺ for biomedical applications.

中文翻译：

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基于高选择性DNA酶的循环形成和金纳米颗粒介导的扩增的电化学测定Ca ²⁺

钙离子（Ca ²⁺）在人体的许多生理和生化过程中起着至关重要的和必不可少的作用。在本报告中，我们展示了一种新型的电化学方法，可通过三种功能性DNA探针和金纳米颗粒（AuNPs）辅助测定Ca ²⁺含量。与其他金属阳离子相比，它在感应Ca ^2+方面具有很高的选择性，这是由于在电极界面处采用了具有极高结合能力的DNAzyme。该方法还整合了DNA酶的循环形成和AuNPs介导的扩增。因此，有望实现高灵敏度。因此，这项工作为生物医学应用提供了一种探测Ca ²⁺的有利方法。