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Eukaryotic RNA 5′-End NAD+ Capping and DeNADding
Trends in Cell Biology ( IF 19.0 ) Pub Date : 2018-03-12 , DOI: 10.1016/j.tcb.2018.02.005
Megerditch Kiledjian

A hallmark of eukaryotic mRNAs has long been the 5′-end m7G cap. This paradigm was recently amended by recent reports that Saccharomyces cerevisiae and mammalian cells also contain mRNAs carrying a novel nicotinamide adenine dinucleotide (NAD+) cap at their 5′-end. The presence of an NAD+ cap on mRNA uncovers a previously unknown mechanism for controlling gene expression through nucleotide metabolite-directed mRNA turnover. In contrast to the m7G cap that stabilizes mRNA, the NAD+ cap targets RNA for rapid decay in mammalian cells through the DXO non-canonical decapping enzyme which removes intact NAD+ from RNA in a process termed ‘deNADding’. This review highlights the identification of NAD+ caps, their mode of addition, and their functional significance in cells.



中文翻译:

真核RNA 5'-末端NAD +封端和脱NADing

真核mRNA的标志一直是5'端m 7 G帽。最近,有关酿酒酵母和哺乳动物细胞也含有在其5'末端带有一个新的烟酰胺腺嘌呤二核苷酸(NAD +)帽的mRNA对该模型进行了修正。NAD +帽在mRNA上的存在揭示了以前未知的机制,该机制可通过核苷酸代谢物控制的mRNA转换来控制基因表达。与稳定mRNA的m 7 G帽相反,NAD +帽通过去除了完整NAD +的DXO非经典去壳酶将RNA靶向哺乳动物细胞中的快速衰变。从RNA中分离出来的过程称为“去NADING”。这篇评论重点介绍了NAD +帽的鉴定,它们的添加方式及其在细胞中的功能意义。

更新日期:2018-03-12
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