Abstract This study developed biocatalytic membranes that can effectively control the surface biofouling based on the enzymatic quenching of bacterial quorum sensing. Acylase (AC) was immobilized and stabilized on intact carbon nanotubes (CNTs) via enzyme adsorption, precipitation, and crosslinking (EAPC) method, maintaining 66% of its initial enzyme activity for 200 days under rigorous shaking (200 rpm). This highly stable EAPC was anchored on the polyvinylidene fluoride (PVDF) microfilter using polydopamine coatings (EAPC membrane). The antifouling performance and mechanism of EAPC membrane was intensively evaluated under static incubation with a model bacterium of Pseudomonas aeruginosa. When compared with the PVDF membranes with and without free AC, the EAPC membrane enhanced the water permeability by 5-folds at an optimum loading of 0.40 g-CNTs/m2 by effectively inhibiting the biofilm formation. The EAPC membrane with this optimal CNT loading did not increase the hydraulic resistance of membrane itself. In the lab-scale continuous filtrations, the EAPC membrane with its loading of 0.40 g-CNTs/m2 demonstrated 1.6-fold delay of trans-membrane pressure increase compared to the PVDF membrane. It is anticipated that biocatalytic membrane based on quorum quenching nanobiocatalysis has a great potential in antifouling applications without changing the process configuration or additional treatments.

中文翻译：

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具有稳定在完整碳纳米管上的酰化酶的生物催化膜通过定额淬火有效防污

摘要 本研究基于细菌群体感应的酶促猝灭，开发了可有效控制表面生物污染的生物催化膜。酰化酶 (AC) 通过酶吸附、沉淀和交联 (EAPC) 方法固定并稳定在完整的碳纳米管 (CNT) 上，在剧烈摇动 (200 rpm) 下保持其初始酶活性的 66% 200 天。这种高度稳定的 EAPC 使用聚多巴胺涂层（EAPC 膜）固定在聚偏二氟乙烯 (PVDF) 微过滤器上。EAPC 膜的防污性能和机理在与铜绿假单胞菌模型细菌静态培养下进行了深入评估。与含和不含游离 AC 的 PVDF 膜相比，EAPC 膜在最佳负载量为 0 时将透水性提高了 5 倍。40 g-CNTs/m2 通过有效抑制生物膜形成。具有这种最佳 CNT 负载的 EAPC 膜不会增加膜本身的水力阻力。在实验室规模的连续过滤中，与 PVDF 膜相比，负载为 0.40 g-CNTs/m2 的 EAPC 膜表现出跨膜压力增加的 1.6 倍延迟。预计基于群体淬灭纳米生物催化的生物催化膜在不改变工艺配置或额外处理的情况下在防污应用中具有巨大潜力。与 PVDF 膜相比，跨膜压力增加延迟了 6 倍。预计基于群体淬灭纳米生物催化的生物催化膜在不改变工艺配置或额外处理的情况下在防污应用中具有巨大潜力。与 PVDF 膜相比，跨膜压力增加延迟了 6 倍。预计基于群体淬灭纳米生物催化的生物催化膜在不改变工艺配置或额外处理的情况下在防污应用中具有巨大潜力。