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A monolithic column based on covalent cross-linked polymer gels for online extraction and analysis of trace aflatoxins in food sample
Journal of Chromatography A ( IF 4.1 ) Pub Date : 2018-03-12 , DOI: 10.1016/j.chroma.2018.03.015
Tianfu Wei , Zhengyi Chen , Gongke Li , Zhuomin Zhang

Aflatoxins are highly toxic mycotoxin contamination, which pose serious food safety incidents. It is very important to precisely and rapidly determine trace aflatoxins in food. In this study, we designed porous monolithic column based on covalent cross-linked polymer gels for online extraction and analysis of trace aflatoxins in food samples with complicated matrices coupled with high-performance liquid chromatography-ultraviolet detector (HPLC-UV). The prepared monolithic column showed excellent enrichment performance due to its good permeability, good reproducibility and long life span. The study of adsorption mechanism suggested that the excellent enrichment performance of this monolithic column was attributed to the multiple effect of π-π stacking interaction, hydrophobic effect and steric effect. When the online analytical method was applied for the determine of trace aflatoxins in real food samples, aflatoxins G1 and aflatoxins B1 could be actually found in one positive bean sauce sample and quantified to be 32.8 and 26.4 μg/kg, respectively. Aflatoxins G1 in one bean sample could be also found and quantified to be 25.9 μg/kg. The low detection limits of the developed method were achieved in range of 0.08-0.2 μg/kg. And the recoveries for spiked samples were in range from 76.1 to 113% with RSDs of 1.1-9.6%. The developed method was proved to be a promising method for online enrichment and analysis of trace aflatoxins in complicated food samples.



中文翻译:

基于共价交联聚合物凝胶的整体柱,用于在线提取和分析食品样品中的痕量黄曲霉毒素

黄曲霉毒素是剧毒的霉菌毒素污染,会造成严重的食品安全事故。准确快速地确定食品中的痕量黄曲霉毒素非常重要。在这项研究中,我们设计了基于共价交联聚合物凝胶的多孔整体柱,用于在线萃取和分析复杂基质食品中的痕量黄曲霉毒素,并结合了高效液相色谱-紫外检测器(HPLC-UV)。制备的整体柱由于其良好的渗透性,良好的重现性和长寿命而显示出优异的富集性能。吸附机理的研究表明,该整体柱的优异富集性能归因于π-π堆积相互作用,疏水作用和位阻作用的多重作用。实际上,在一个阳性豆沙酱样品中实际上发现了1和黄曲霉毒素B 1,其定量分别为32.8和26.4μg/ kg。还可以在一个豆子样品中发现黄曲霉毒素G 1并定量为25.9μg/ kg。所开发方法的低检出限在0.08-0.2μg/ kg的范围内。加标样品的回收率在76.1%至113%之间,RSD为1.1-9.6%。实践证明,所开发的方法是在线富集和分析复杂食品中痕量黄曲霉毒素的有前途的方法。

更新日期:2018-03-12
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