Low energy ultrasound irradiation was used to enhance co-production of enzymes uricase and alkaline protease using Bacillus licheniformis NRRL 14209. Production of uricase and alkaline protease were evaluated for different ultrasound parameters such as ultrasound power, time of irradiation, duty cycle and growth stage of organisms at which irradiation is carried out. Maximum uricase production of 0.825 U/mL and alkaline protease of 0.646 U/mL have been obtained when fermentation broth was irradiated at 6 h of growth stage with 60 W power for 15 min of duration having 40% of duty cycle. The enzyme yield was found to be enhanced by a factor of 1.9 – 3.8 and 1.2 - 2.2 for uricase and alkaline protease respectively. Nevertheless, intracellular uricase was also observed in a fermentation broth after ultrasonic process intensification. The results indicate the effectiveness of low frequency ultrasound in improving enzyme yields with a vision of commercial applicability of the process.

低能量超声照射用于使用地衣芽孢杆菌增强尿酸酶和碱性蛋白酶的协同生产NRRL 14209.对尿素和碱性蛋白酶的生产进行了评估，以了解不同的超声参数，例如超声功率，辐照时间，工作周期和进行辐照的生物的生长阶段。当在生长阶段的6小时内以60 W功率照射发酵液持续15分钟，占空比为40％时，最大尿酸酶产量为0.825 U / mL，碱性蛋白酶为0.646 U / mL。发现尿酶和碱性蛋白酶的酶产量分别提高了1.9 – 3.8和1.2 – 2.2。然而，在超声过程强化后的发酵液中也观察到了细胞内尿酸酶。结果表明，鉴于该方法的商业适用性，低频超声在提高酶产率方面的有效性。