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Immobilizing Siderophores on Solid Surfaces for Bacterial Detection
Journal of The Electrochemical Society ( IF 3.9 ) Pub Date : 2018-03-12 00:00:00 , DOI: 10.1149/2.0041808jes
Priyanka Bhadra 1 , Mohammed S. Shajahan 1 , Paresh N. Patel 2 , Enakshi Bhattacharya 1, 3 , Anju Chadha 1, 2, 4 , Praveen Kumar Sekhar 5
Affiliation  

The article reports a proof of concept validation of bacterial detection using Siderophores. Desferrioxamine B (DesfB) was used as the Siderophore to capture Escherichia coli on gold coated microcantilever surface. Self-assembled monolayer based gold thiol chemistry was used for surface functionalization of the Siderophore on top surface of the microcantilever. The bacterial attachment to the siderophore was observed through Fourier Transform Infrared Spectroscopy (FTIR) and Fluorescence Microscopy. The DesfB coated surface allows only the live bacterial cells to be attached and it is evident through the FTIR band formation. In a mixture of live and dead E. coli cells, the Fluorescence Microscope image indicates green emission from live cells and a core-shell structure formation upon progress of time. For a sample dilution of 10−1, the mass change of live E. coli bonded to Siderophores is four times higher than that of dead cells and 12 times higher to that of negative control on microcantilevers. Therefore this study should be considered as a foundation to build a miniaturized biosensing platform to distinguish between bacterial or viral infections in real time. The proposed platform could differentiate between bacterial and viral infections thus rendering it as Point of Care (PoC) diagnostic tool aiding Internet of Things (IoT) applications.

中文翻译:

将铁载体固定在固体表面上以进行细菌检测

这篇文章报告了使用Siderophores进行细菌检测的概念验证。Desferrioxamine B(DesfB)用作铁载体,可在包金的微悬臂梁表面捕获大肠杆菌。基于自组装单层的金硫醇化学用于微悬臂梁顶表面上的铁载体的表面功能化。通过傅立叶变换红外光谱(FTIR)和荧光显微镜观察到细菌附着在铁载体上。涂有DesfB的表面仅允许附着活细菌细胞,这通过FTIR谱带的形成是显而易见的。在活的和死的大肠杆菌中混合细胞,荧光显微镜图像显示随着时间的流逝,活细胞发出绿色光,并形成核-壳结构。对于10 -1的样品稀释度,结合到Siderophores上的活大肠杆菌的质量变化是死细胞的质量变化的四倍,是微悬臂梁上的阴性对照的质量变化的12倍。因此,该研究应被视为构建微型生物传感平台以实时区分细菌或病毒感染的基础。拟议的平台可以区分细菌感染和病毒感染,从而使其成为辅助物联网(IoT)应用程序的即时诊断(PoC)诊断工具。
更新日期:2018-03-12
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