A novel application of nanofiber polymers in the wide area of a sample preparation techniques known as solid phase extraction has been studied. We demonstrated application of nanofibers as sorbents for use in a system including on-line extraction coupled with column-switching high performance liquid chromatography. Four types of nanofibers including polyamide 6 with two different surface densities, poly(ε-caprolactone), and polystyrene were tested. We found that three of them were very efficient extraction sorbents sufficiently stable for application in the on-line system. Our results confirmed that the extraction efficiency of the nanofibers depended on the type and chemistry of the spun polymer as well as on the fabrication process of the nanofibrous mats that affected their density, structure, surface density, and mechanical functionality. We also compared performance of all four nanofibers with a conventional monolithic reversed-phase C18 sorbent in terms of extraction efficiency using on-line solid-phase extraction-HPLC system. Polyamide 6 was found to be the best sorbent for lipophilic analytes with a retention and extraction efficiency for the target analytes comparable with that of the C18 monolith.

中文翻译：

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纳米纤维聚合物作为色谱系统中在线固相萃取的新型吸附剂：与整体反相 C18 吸附剂的比较

已经研究了纳米纤维聚合物在称为固相萃取的样品制备技术的广泛领域中的新应用。我们展示了纳米纤维作为吸附剂在一个系统中的应用，该系统包括在线萃取与柱切换高效液相色谱相结合。测试了四种类型的纳米纤维，包括具有两种不同表面密度的聚酰胺 6、聚（ε-己内酯）和聚苯乙烯。我们发现其中三种是非常有效的萃取吸附剂，其稳定性足以用于在线系统中。我们的结果证实，纳米纤维的提取效率取决于纺丝聚合物的类型和化学性质，以及影响其密度、结构、表面密度和机械功能的纳米纤维垫的制造工艺。我们还使用在线固相萃取-HPLC 系统在萃取效率方面比较了所有四种纳米纤维与传统整体反相 C18 吸附剂的性能。发现聚酰胺 6 是亲脂性分析物的最佳吸附剂，其对目标分析物的保留和萃取效率与 C18 整料相当。