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Aptamer-based microcantilever-array biosensor for profenofos detection
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2018-08-01 , DOI: 10.1016/j.aca.2018.02.072
Chao Li , Guangping Zhang , Shangquan Wu , Qingchuan Zhang

Profenofos, a highly poisonous organophosphorus pesticide, has been widely used in agricultural production. These pesticide residues have seriously influenced food security and threatened human health, and new methods with high sensitivity are greatly needed to detect profenofos. Here, we developed an aptamer-based microcantilever-array sensor operated in stress mode to detect profenofos, with advantages of being a label-free, highly sensitive, one-step immobilization method capable of quantitative and real-time detection. The microcantilevers were functionalized with a profenofos-specific aptamer (SS2-55), and then the specific binding of profenofos to aptamer induced a deflection of the microcantilever, which was monitored using an optical method in a real-time manner. The microcantilever deflection showed a positive relationship with profenofos concentration, and the detection limit was low to 1.3 ng mL-1 (3.5 nM) for profenofos, which was much lower than other aptamer-based detection methods. The selectivity of the sensor was verified with another organophosphorus pesticide. Additionally, we successfully detected profenofos dissolved in vegetable-soak solution. Our results showed that this aptamer-based microcantilever-array sensor is a convenient and label-free method for detecting profenofos in small amounts and has great potential for food-security applications.

中文翻译:

用于profenofos检测的基于适体的微悬臂梁阵列生物传感器

Profenofos是一种剧毒的有机磷农药,在农业生产中得到了广泛的应用。这些农药残留严重影响了粮食安全,威胁着人类健康,迫切需要新的高灵敏度检测方法来检测丙磺磷。在这里,我们开发了一种基于适体的微悬臂梁阵列传感器,在应力模式下运行以检测 profenofos,具有无标记、高灵敏度、一步固定化的优点,能够进行定量和实时检测。微悬臂梁用 profenofos 特异性适体 (SS2-55) 功能化,然后 profenofos 与适体的特异性结合引起微悬臂梁的偏转,使用光学方法实时监测。微悬臂梁偏转与 profenofos 浓度呈正相关,profenofos 的检测限低至 1.3 ng mL-1 (3.5 nM),远低于其他基于适体的检测方法。传感器的选择性用另一种有机磷农药进行了验证。此外,我们成功检测到溶解在蔬菜浸泡液中的丙磺诺磷。我们的结果表明,这种基于适体的微悬臂梁阵列传感器是一种方便且无标记的方法,可用于检测少量丙磺诺磷,在食品安全应用方面具有巨大潜力。我们成功地检测到溶解在蔬菜浸泡液中的丙磺诺磷。我们的结果表明,这种基于适体的微悬臂梁阵列传感器是一种方便且无标记的方法,可用于检测少量丙磺诺磷,在食品安全应用方面具有巨大潜力。我们成功地检测到溶解在蔬菜浸泡液中的丙磺诺磷。我们的结果表明,这种基于适体的微悬臂梁阵列传感器是一种方便且无标记的方法,可用于检测少量丙磺诺磷,在食品安全应用方面具有巨大潜力。
更新日期:2018-08-01
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