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Ratiometric quantitation of thiol metabolites using non-isotopic mass tags
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2018-12-01 , DOI: 10.1016/j.aca.2018.02.057
Xiaofeng Zhao , Dawn S. Hui , Richard Lee , James L. Edwards

Ratiometric quantitation is used in mass spectrometry to account for variations in ionization efficiencies due to heterogenous sample matrixes. Isotopes are most commonly used to achieve ratiometric quantitation because of their ability to co-elute chromatographically with each other and to have similar ionization efficiencies. In the work presented here, a new non-isotopic quantitative tagging approach is presented which allows chromatographic co-elution and similar ionization efficiencies. Using two variations of maleimide tags, t-butyl and cyclohexyl maleimide, thiols are quantified with a high degree of linearity up to five-fold concentration differences. Because these two tags have similar hydrophobcities, they elute simultaneously which allows them to be used for ratiometric quantitation. Beyond the five-fold linear range, signal compression is observed. This technique was able to quantify thiol changes in both in vitro pharmacological treatments as well as in vivo diabetic tissue.

中文翻译:

使用非同位素质量标签对硫醇代谢物进行比例定量

质谱中使用比率定量来解释由于异质样品基质引起的电离效率的变化。同位素最常用于实现比率定量,因为它们能够在色谱上相互共洗脱并且具有相似的电离效率。在这里介绍的工作中,提出了一种新的非同位素定量标记方法,它允许色谱共洗脱和类似的电离效率。使用马来酰亚胺标签的两种变体,叔丁基和环己基马来酰亚胺,硫醇以高达五倍的浓度差异的高度线性进行量化。由于这两个标签具有相似的疏水性,它们同时洗脱,这使得它们可用于比率定量。超出五倍线性范围,观察到信号压缩。该技术能够量化体外药理治疗和体内糖尿病组织中硫醇的变化。
更新日期:2018-12-01
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