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Exploring the interaction of silver nanoparticles with pepsin and its adsorption isotherms and kinetics
Chemico-Biological Interactions ( IF 5.1 ) Pub Date : 2018-03-10 , DOI: 10.1016/j.cbi.2018.03.004
Xiangrong Li , Kaiwei Wang , Yanru Peng

The interaction of nanoparticles (NPs) with proteins is a topic of high relevance for the medical application of nanomaterials. In the study, a comprehensive investigation was performed for the binding properties of silver nanoparticles (AgNPs) to pepsin. The results indicate that the binding of AgNPs to pepsin may be a static quenching mechanism. Thermodynamic analysis reveals that AgNPs binds to pepsin is synergistically driven by enthalpy and entropy, and the major driving forces are hydrophobic and electrostatic interactions. Synchronous fluorescence spectroscopy shows that AgNPs may induce microenvironmental changes of pepsin. The hydrophobicity of Trp is increased while the hydrophility of Tyr is increased. The adsorption of pepsin on AgNPs was analyzed by Langmuir and Freundlich models, suggesting that the equilibrium adsorption data fit well with Freundlich model. The equilibrium adsorption data were modeled using the pseudo-first-order and pseudo-second-order kinetic equations. The results indicate that pseudo-second-order kinetic equation better describes the adsorption kinetics. The study provides an accurate and full basic data for clarifying the binding mechanism, adsorption isotherms and kinetic behaviors of AgNPs with pepsin. These fundamental works will provide some new insights into the safe and effective application of AgNPs in biological and medical areas.



中文翻译:

探索银纳米颗粒与胃蛋白酶的相互作用及其吸附等温线和动力学

纳米粒子(NPs)与蛋白质的相互作用是与纳米材料医学应用高度相关的主题。在这项研究中,对银纳米颗粒(AgNPs)与胃蛋白酶的结合特性进行了全面研究。结果表明,AgNPs与胃蛋白酶的结合可能是一种静态猝灭机制。热力学分析表明,AgNPs与胃蛋白酶结合是由焓和熵协同驱动的,主要驱动力是疏水和静电相互作用。同步荧光光谱表明,AgNPs可能诱导胃蛋白酶的微环境变化。Trp的疏水性增加,而Tyr的亲水性增加。胃蛋白酶在AgNPs上的吸附通过Langmuir和Freundlich模型进行了分析,这表明平衡吸附数据与Freundlich模型非常吻合。平衡吸附数据使用拟一级动力学方程和拟二级动力学方程建模。结果表明,伪二级动力学方程更好地描述了吸附动力学。该研究为阐明AgNPs与胃蛋白酶的结合机理,吸附等温线和动力学行为提供了准确而完整的基础数据。这些基础性工作将为在农业和生物领域安全有效地应用AgNPs提供一些新的见解。该研究为阐明AgNPs与胃蛋白酶的结合机理,吸附等温线和动力学行为提供了准确而完整的基础数据。这些基础性工作将为在农业和生物领域安全有效地应用AgNPs提供一些新的见解。该研究为阐明AgNPs与胃蛋白酶的结合机理,吸附等温线和动力学行为提供了准确而完整的基础数据。这些基础性工作将为在农业和生物领域安全有效地应用AgNPs提供一些新的见解。

更新日期:2018-03-10
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