The current study was aimed to investigate the detoxifying and antioxidant enzyme response of Gammarus pulex exposed to malachite green (MG) after decolorization by Coriolus versicolor. Response surface methodology (RSM) was utilized to optimize the decolorization conditions of MG synthetic solutions by C. versicolor. Glutathione (GSH), malondialdehyde (MDA) levels and glutathione peroxidase (GP_X), catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), cytochrome P450 1A1 (CYP1A1) activities in G. pulex exposed to undecolorized (A1) and decolorized (A2) MG synthetic solution during 24 and 96 h were tested by using ELISA method. SOD and GP_X enzyme activity was increased after decolorization (p > 0.05). CAT enzyme activity was increased in A2 group during 24 h (p > 0.05) but decreased during 96 h (p < 0.05). GSH levels were increased in A2 group during 24 and 96 h (p < 0.05). GST, CYP1A1 enzyme activity and MDA levels were decreased after decolorization during 96 h (p < 0.05). In this study, GSH levels, CAT, GST and CYP1A1 activities in G. pulex approved the capability of C. versicolor in MG decolorization, optimized with RSM.

目前的研究旨在研究在暴露于孔雀绿（Coriolus versicolor）脱色的孔雀绿（MG）后的γpulex pulex的解毒和抗氧化酶反应。响应面法（RSM）用于优化C. versicolor对MG合成溶液的脱色条件。暴露于G.pulex的G.pulex中的谷胱甘肽（GSH），丙二醛（MDA）水平和谷胱甘肽过氧化物酶（GP _X），过氧化氢酶（CAT），超氧化物歧化酶（SOD），谷胱甘肽S-转移酶（GST），细胞色素P450 1A1（CYP1A1）活性使用ELISA方法检测24和96小时内未脱色（A1）和脱色（A2）的MG合成溶液。SOD和GP _X脱色后酶活性增加（p> 0.05）。CAT酶活性在A2组中在24小时内增加（p> 0.05），但在96小时内降低（p <0.05）。A2组在24和96 h内GSH水平升高（p <0.05）。脱色96小时后，GST，CYP1A1酶活性和MDA水平降低（p <0.05）。在这项研究中，GSH水平，CAT，GST中CYP1A1和活动G.蚤批准的能力云芝在MG脱色，用RSM优化。