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Rapid detection of single E. coli bacteria using a graphene-based field-effect transistor device
Biosensors and Bioelectronics ( IF 12.6 ) Pub Date : 2018-03-09 , DOI: 10.1016/j.bios.2018.03.014
Bhawana Thakur , Guihua Zhou , Jingbo Chang , Haihui Pu , Bing Jin , Xiaoyu Sui , Xiaochen Yuan , Ching-Hong Yang , Matthew Magruder , Junhong Chen

Contamination of surface and drinking water due to the presence of Escherichia coli bacteria is a major cause of water-borne disease outbreak. To address unmet challenges for practical pathogen detection in contaminated samples, we report fabrication of thermally reduced graphene oxide-based field-effect transistor (rGO FET) passivated with an ultrathin layer of Al2O3 for real-time detection of E. coli bacteria. The sensor could detect a single E. coli cell within 50 s in a 1 µL sample volume. The ultrathin layer of Al2O3 acted as a barrier between rGO and potential interferents present in the sample. E. coli specific antibodies anchored on gold nanoparticles acted as probes for selective capture of E. coli. The high density of negative charge on the surface of E. coli cells strongly modulates the concentration of majority charge carriers in the rGO monolayer, thereby allowing real-time monitoring of E. coli concentration in a given sample. With a low detection limit of single cell, the FET sensor had a linear range of 1–100 CFU in 1 µL volume of sample (i.e., 103 to 105 CFU/ mL). The biosensor with good selectivity and rapid detection was further successfully demonstrated for E. coli sensing in river water. The rGO-based FET sensor provides a low cost and label-free approach, and can be mass produced for detection of a broad spectrum of pathogens in water or other liquid media.



中文翻译:

使用基于石墨烯的场效应晶体管器件快速检测单个大肠杆菌

由于存在大肠埃希氏菌而污染了地表水和饮用水,是造成水传播疾病暴发的主要原因。为了解决污染的样品在实际病原体检测未满足的挑战,我们报告热还原的石墨烯氧化物基场效应晶体管的制造(RGO FET)与Al的超薄层钝化2 ø 3用于实时检测大肠杆菌菌。该传感器可以在1秒内以1 µL的体积检测到一个大肠杆菌细胞。Al 2 O 3的超薄层充当rGO与样品中潜在干扰物之间的屏障。大肠杆菌锚定在金纳米颗粒上的特异性抗体作为选择性捕获大肠杆菌的探针。大肠杆菌细胞表面高密度的负电荷会强烈调节rGO单层中多数电荷载体的浓度,从而可以实时监控给定样品中的大肠杆菌浓度。FET传感器的单电池检测限较低,在1 µL样品体积中(即10 3至10 5 CFU / mL)具有1–100 CFU的线性范围。具有良好选择性和快速检测能力的生物传感器进一步成功地用于大肠杆菌在河水中感应。基于rGO的FET传感器提供了一种低成本且无标签的方法,可以批量生产以检测水或其他液体介质中的多种病原体。

更新日期:2018-03-09
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