This study evaluated the effect of QDs functionalized with the antimicrobial peptide indolicidin on oxidative stress and metabolomics profiles of Daphnia magna across three generations (F0, F1, and F2). Exposing D. magna to sub-lethal concentrations of the complex QDs-indolicidin, a normal survival of daphnids was observed from F0 to F2, but a delay of first brood, fewer broods per female, a decrease of length of about 50% compared to control. In addition, QDs-indolicidin induced a significantly higher production of reactive oxygen species (ROS) gradually in each generation and an impairment of enzymes response to oxidative stress such as superoxide dismutase (SOD), catalase (CAT) and glutathione transferase (GST). Effects were confirmed by metabolomics profiles that pointed out a gradual decrease of metabolomics content over the three generations and a toxic effect of QDs-indolicidin likely related to the higher accumulation of ROS and decreased antioxidant capacity in F1 and F2 generations. Results highlighted the capability of metabolomics to reveal an early metabolic response to stress induced by environmental QDs-indolicidin complex.

这项研究评估了用抗菌肽吲哚美定功能化的QD对三代水蚤（Daphnia magna）的氧化应激和代谢组学谱的影响（F0，F1和F2）。暴露D. magna到复合致死量的QDs-吲哚啶菌素的亚致死浓度以下，观察到了ph从F0到F2的正常存活，但是与对照相比，第一亲鱼延迟了，每只雌性的亲鱼减少了，长度减少了约50％。此外，QDs-吲哚丁啶在每一代中逐渐诱导显着更高的活性氧（ROS）产生，并削弱了对氧化应激的酶的响应，例如超氧化物歧化酶（SOD），过氧化氢酶（CAT）和谷胱甘肽转移酶（GST）。代谢组学资料证实了这一作用，指出了三代代谢组学含量的逐渐降低以及QDs-吲哚丁素的毒性作用可能与ROS的较高积累和F1和F2世代的抗氧化能力下降有关。