Abstract Synthesis of iron oxide nanoparticles has attracted increasing interest due to their importance in biomedical and technological applications. To investigate the ability of Juglans regia (J. regia) green husk extract in iron oxide nanoparticles size control, they were synthesized through co-precipitation method with J. regia extract and without it. For both tests, other experimental conditions were same. According to High-resolution transmission electron microscopy, the mean diameter and standard deviation of iron oxide nanoparticles and J. regia/iron oxide nanoparticles synthesized using co-precipitation method were 12.60 ± 2.87 and 5.77 ± 1.66 nm respectively. These results showed that J. regia/iron oxide nanoparticles synthesized using extract have a smaller size than nanoparticles fabricated by co-precipitation method; moreover, green husk extract plays the main role as size control. The obtained results of Powder X-ray diffraction (PXRD), High resolution transmission electron microscopy (HR-TEM), Field emission scanning electron microscopy (FESEM), and Energy dispersive X-ray (EDX) are in good agreement with each other and confirm the high purity of fabricated magnetic nanoparticles using J. regia extract. Vibrating sample magnetometer (VSM) revealed that the J. regia/iron oxide nanoparticles due to their proper magnetic properties have high saturation magnetization and low coercivity. According to FTIR spectrum, the J. regia would be coated on the iron oxide nanoparticles surface in a successful manner. The non-toxic effect of J. regia/iron oxide nanoparticles concentration below 1000 µg/ml was observed in the studies of in vitro cytotoxicity on normal and cancerous cell lines, respectively. The dose-dependent toxicity made it a suitable candidate for various medical applications.

中文翻译：

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使用胡桃绿皮提取物对植物介导的合成氧化铁纳米颗粒的细胞毒性测定

摘要氧化铁纳米粒子的合成由于其在生物医学和技术应用中的重要性而引起了越来越多的兴趣。为了研究胡桃（J. regia）绿皮提取物在氧化铁纳米颗粒尺寸控制中的能力，它们通过与王权提取物和没有它的共沉淀法合成。对于这两个测试，其他实验条件相同。根据高分辨率透射电子显微镜，共沉淀法合成的氧化铁纳米颗粒和王浆/氧化铁纳米颗粒的平均直径和标准偏差分别为 12.60 ± 2.87 和 5.77 ± 1.66 nm。这些结果表明，与共沉淀法制备的纳米颗粒相比，使用提取物合成的王浆/氧化铁纳米颗粒具有更小的尺寸；而且，绿皮提取物主要起控制尺寸的作用。粉末 X 射线衍射 (PXRD)、高分辨透射电子显微镜 (HR-TEM)、场发射扫描电子显微镜 (FESEM) 和能量色散 X 射线 (EDX) 所得结果相互吻合，使用王权提取物确认制造的磁性纳米粒子的高纯度。振动样品磁强计 (VSM) 表明，王水/氧化铁纳米颗粒由于其适当的磁性而具有高饱和磁化强度和低矫顽力。根据 FTIR 光谱，王水可以成功地涂覆在氧化铁纳米颗粒表面。J的无毒作用 在分别对正常和癌细胞系的体外细胞毒性研究中，分别观察到 regia/氧化铁纳米颗粒浓度低于 1000 µg/ml。剂量依赖性毒性使其成为各种医学应用的合适候选者。