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Au–Se-Bond-Based Nanoprobe for Imaging MMP-2 in Tumor Cells under a High-Thiol Environment
Analytical Chemistry ( IF 7.4 ) Pub Date : 2018-03-09 00:00:00 , DOI: 10.1021/acs.analchem.7b05343
Xiaonan Gao 1 , Lulu Jiang 1 , Bo Hu 1 , Fanpeng Kong 1 , Xiaojun Liu 1 , Kehua Xu 1 , Bo Tang 1
Affiliation  

The gold nanosensors based on the Au–S bond have been widely applied to biochemical detections. However, signal distortion caused by biothiols has been seldom mentioned and urgently needs to be solved. Herein, we designed a novel but easily assembled gold nanoprobe by coupling a selenol-modified peptide with FITC onto the gold nanoparticle’s surface via an Au–Se bond for fluorescence imaging of a tumor marker matrix, metalloproteinases 2 (MMP-2). Compared to the Au–S probes, the Au–Se probes display high thermal stability and a very good anti-interference ability toward glutathione under simulated physiological conditions. More importantly, the Au–Se nanoprobe exhibits a high-fidelity fluorescent signal toward MMP-2, effectively avoiding interference caused by high levels of thiol compounds in vivo. In addition, in vivo experiments further proved that no significant signal intensity change for the tumor cells treated by the Au–Se probes was observed before and after eliminating glutathione. Hence, we believe such Au–Se probes with in vivo glutathione interfering resistance offer new routes and perspectives in biology and medicine in the future.

中文翻译:

基于金-硒键的纳米探针在高硫醇环境下成像肿瘤细胞中的MMP-2

基于Au–S键的金纳米传感器已广泛应用于生化检测。但是,很少提及由生物硫醇引起的信号失真,因此迫切需要解决。在这里,我们设计了一种新颖但易于组装的金纳米探针,方法是通过Au-Se键将硒醇修饰的肽与FITC偶联到金纳米颗粒的表面,用于肿瘤标记物基质金属蛋白酶2(MMP-2)的荧光成像。与Au–S探针相比,Au–Se探针在模拟的生理条件下显示出高的热稳定性和对谷胱甘肽的很好的抗干扰能力。更重要的是,Au-Se纳米探针对MMP-2表现出高保真荧光信号,有效避免了体内高水平硫醇化合物引起的干扰。此外,体内实验进一步证明,在消除谷胱甘肽之前和之后,经Au-Se探针处理的肿瘤细胞均未观察到明显的信号强度变化。因此,我们相信这种具有体内谷胱甘肽干扰抗性的Au-Se探针在将来为生物学和医学提供了新的途径和观点。
更新日期:2018-03-09
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