Conjugation of fluorescent polymer chains synthesized by RAFT controlled radical polymerization with native proteins was achieved by introducing an activated ester function at their ω-chain-end via a very efficient thiol–ene click post-polymerization functionalization strategy. These fluorescent polymer probes covalently reacted with the lysine amino groups of native proteins to form bright protein–polymer fluorescent conjugates. In particular, streptavidin was labeled with various polymer probes and it was shown that it retained in each case its ability to selectively bind biotinylated compounds. Finally, streptavidin labeled with fluorescent polymer chains was used to reveal biotinylated antibodies, enabling the detection of CD40 receptors at the surface of live dendritic cells.

中文翻译：

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用荧光RAFT聚合物探针标记天然蛋白：应用流式细胞仪检测细胞表面蛋白†

通过非常有效的硫醇-烯点击后聚合功能化策略，通过RAFT控制的自由基聚合合成的荧光聚合物链与天然蛋白质的缀合是通过在其ω-链末端引入活化的酯官能团实现的。这些荧光聚合物探针与天然蛋白质的赖氨酸氨基共价反应，形成明亮的蛋白质-聚合物荧光偶联物。特别地，抗生蛋白链菌素用各种聚合物探针标记，并且显示在每种情况下它都保留了其选择性结合生物素化化合物的能力。最后，用荧光聚合物链标记的链霉亲和素被用于揭示生物素化抗体，从而能够检测活的树突状细胞表面的CD40受体。