Centrifugal microfluidics or lab-on-a-disc (LOAD) is a promising branch of lab-on-a-chip or microfluidics. Besides effective fluid transportation and inherently available density-based sample separation in centrifugal microfluidics, uniform actuation of flow on the disc makes the platform compact and scalable. However, the natural radially outward centrifugal force in a LOAD system limits its capacity to perform complex fluid manipulation steps. In order to increase the fluid manipulation freedom and integration capacity of the LOAD system, we propose a binary centrifugal microfluidics platform. With the help of Euler force, our platform allows free switching of both left and right states based on a rather simple mechanical structure. The periodical switching of state would provide a “clock” signal for a sequence of droplet binary logic operations. With the binary state platform and the “clock” signal, we can accurately handle the droplet separately in each time step with a maximum main frequency of about 10 S s⁻¹ (switching per second). Apart from droplet manipulations such as droplet generation and metering, we also demonstrate a series of droplet logic operations, such as binary valving, droplet routing and digital addressable droplet storage. Furthermore, complex bioassays such as the Bradford assay and DNA purification assay are demonstrated on a binary platform, which is totally impossible for a traditional LOAD system. Our binary platform largely improves the capability for logic operation on the LOAD platform, and it is a simple and promising approach for microfluidic lab-on-a-disc large-scale integration.

中文翻译：

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二进制离心微流控技术可实现新颖的数字寻址功能，用于阀门和选路†

离心微流控技术或片上实验室（LOAD）是芯片实验室或微流控技术的一个有前途的分支。除了有效的流体传输和离心微流体中固有的基于密度的样品分离之外，圆盘上的均匀流驱动使平台紧凑且可扩展。但是，LOAD系统中的自然径向向外离心力会限制其执行复杂的流体操作步骤的能力。为了增加LOAD系统的流体操纵自由度和集成能力，我们提出了一种二元离心微流控平台。在欧拉力的帮助下，我们的平台允许基于相当简单的机械结构自由切换左右状态。状态的周期性切换将为微滴二进制逻辑操作序列提供“时钟”信号。^-1（每秒切换）。除了液滴操作（例如液滴生成和计量）外，我们还演示了一系列液滴逻辑操作，例如二进制阀，液滴路由和数字可寻址液滴存储。此外，在二元平台上演示了复杂的生物测定法，例如Br​​adford测定法和DNA纯化测定法，这对于传统的LOAD系统是完全不可能的。我们的二进制平台极大地提高了LOAD平台上逻辑运算的能力，并且它是用于微流体盘上实验室大规模集成的一种简单而有希望的方法。