A microbioreactor immobilized with a synthase-type mutant enzyme, Endo-M-N175Q (glycosynthase) of endo-β-N-acetylglucosaminidase derived from Mucor hiemalis (Endo-M), was constructed and used for glycoconjugate synthesis. The transglycosylation was performed with a reaction mixture containing an oxazoline derivative of sialo complex-type glycoside (SG), which was prepared from a sialo complex-type glycopeptide SGP derived from hen egg yolk, as a glycosyl donor and N-Fmoc-N-acetylglucosaminyl-l-asparagine [Fmoc-Asn(GlcNAc)-OH] as an acceptor. The reaction mixture was injected into a glycosynthase microbioreactor at a constant flow rate. Highly efficient and nearly stoichiometric transglycosylation occurred in the microbioreactor, and the transglycosylation product was eluted from the other end of the reactor. The glycosynthase microbioreactor was stable and could be used repeatedly for a long time.

构建了固定有合酶型突变酶的微生物生物反应器，该酶是来源于Mucor hiemalis（Endo-M）的内-β- N-乙酰氨基葡糖苷酶的Endo-M-N175Q（糖合成酶），并用于糖缀合物的合成。转糖用含唾液酸复合型糖苷（SG），其从母鸡蛋黄衍生的唾液酸复合型糖肽SGP制备的恶唑啉衍生物的反应混合物中进行，作为糖供体和Ñ -Fmoc- ñ - acetylglucosaminyl-升-天冬酰胺[Fmoc-Asn（GlcNAc）-OH]作为受体。将反应混合物以恒定流速注入糖合酶微生物反应器中。在微生物反应器中发生了高效且化学计量接近的转糖基化反应，并且从反应器的另一端洗脱了转糖基化产物。糖合酶微生物反应器是稳定的，可以长时间重复使用。