In this work, electrically-conducting poly(Toludine Blue) was employed for the first time as synthetic receptor film, prepared by Molecular Imprinting strategies and using electrochemical methods, for the specific screening of breast cancer biomarker Carbohydrate Antigen 15-3 (CA 15-3).

The protein imprinted poly(Toluidine Blue) film was grown in a pre-formed Toluidine Blue (TB) tailed SAM at the AuSPE surface, which greatly enhanced the stability against degradation of the Molecular Imprinted Polymer (MIP) film at the electrode surface.

The MIP receptor film recognition ability towards the protein was investigated by fitting data to Freundlich isotherm. The binding affinity (K_F) obtained for the MIP system was significantly higher (~ 12-fold) to that obtained for the NIP system, demonstrating the success of the approach in creating imprinted materials that specifically respond to CA 15-3 protein.

The incubation of the MIP modified electrode with increasing concentration of protein (from 0.10 U mL⁻¹ to 1000 U mL⁻¹) resulted in a decrease of the ferro/ferricyanide redox current. The device displayed linear response from 0.10 U mL⁻¹ to 100 U mL⁻¹ and LODs below 0.10 U mL⁻¹ were obtained from calibration curves built in neutral buffer and diluted artificial serum, using DPV technique, enabling the detection of the protein biomarker at clinically relevant levels. The developed MIP biosensor was applied to the determination of CA 15-3 in spiked serum samples with satisfactory results.

The developed device provides a new strategy for sensitive, rapid, simple and cost-effective screening of CA 15-3 biomarker. Importantly, the overall approach seems suitable for point-of-care (PoC) use in clinical context.

在这项工作中，导电聚甲苯胺蓝首次被用作合成受体膜，通过分子印迹策略和电化学方法制备，用于特异性筛选乳腺癌生物标志物碳水化合物抗原 15-3 (CA 15- 3）。

蛋白质印迹聚（甲苯胺蓝）薄膜在 AuSPE 表面的预先形成的甲苯胺蓝（TB）尾 SAM 中生长，这极大地增强了电极表面分子印迹聚合物（MIP）薄膜降解的稳定性。

通过将数据拟合到 Freundlich 等温线来研究 MIP 受体膜对蛋白质的识别能力。MIP 系统获得的结合亲和力 (K _F ) 显着高于 NIP 系统获得的结合亲和力 (~ 12 倍)，证明该方法成功地创建了对 CA 15-3 蛋白有特异性反应的印迹材料。

随着蛋白质浓度的增加（从0.10 U mL ^-1到1000 U mL ^-1），MIP修饰电极的孵育导致亚铁/铁氰化物氧化还原电流降低。^{该装置显示出从 0.10 U mL -1}到 100 U mL ^-1的线性响应，并且使用 DPV 技术从在中性缓冲液和稀释的人工血清中构建的校准曲线获得低于 0.10 U mL ^-1的LOD ，从而能够检测蛋白质生物标志物在临床相关水平。开发的MIP生物传感器应用于加标血清样品中CA 15-3的测定，结果令人满意。

开发的设备为 CA 15-3 生物标志物的灵敏、快速、简单和经济高效的筛选提供了一种新策略。重要的是，整体方法似乎适合在临床环境中使用即时护理 (PoC)。