A new UHPLC-DAD-MS method based on a Core-Shell particles column was developed to realize the rapid separation of saffron stigma metabolites (Crocus sativus L.). A single separation of 35 compounds included cis and trans-crocetin esters (crocins), cis-crocetin, trans-crocetin, kaempferol derivatives, safranal, and picrocrocin from pure saffron stigmas. This method permitted the detection of 11 picrocrocin derivatives as the typical group of compounds from saffron as well as the detection of gardenia-specific compounds as typical adulterant markers. The metabolite concentration in a Standardized Saffron Extract (SSE) was determined using the method described herein and by comparison to the ISO3632 conventional method. The safranal content was 5–150 times lower than the value of 2% that was expected via ISO3632 analyses. Using the same Core-Shell separation, geniposide detection appeared to be a relevant approach for detecting the adulteration of saffron by using gardenia.

开发了一种基于核-壳颗粒色谱柱的新的UHPLC-DAD-MS方法，以实现藏红花柱头代谢物（Crocus sativus L.）的快速分离。35种化合物的单一分离物包括顺式和反式-crocetin酯（crocins），顺式-crocetin，反式-藏红花柱头中的-crocetin，山奈酚衍生物，safranal和picrocrocin。该方法允许从藏红花中检测出11种微红霉素衍生物作为典型的化合物组，并检测出ga​​rden子特定的化合物作为典型的掺假标记。使用本文描述的方法并与ISO3632常规方法进行比较，确定标准藏红花提取物（SSE）中的代谢物浓度。sa中的含量比通过ISO3632分析预期的2％的值低5–150倍。使用相同的核-壳分离，子苷检测似乎是通过使用garden子检测藏红花掺假的一种相关方法。