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Biophysical Assessment of Pulmonary Surfactant Predicts the Lung Toxicity of Nanomaterials
Small Methods ( IF 12.4 ) Pub Date : 2018-03-07 , DOI: 10.1002/smtd.201700367
Yi Yang 1 , Yakun Wu 2, 3 , Quanzhong Ren 2, 3 , Lijie Grace Zhang 4 , Sijin Liu 2, 3 , Yi Y. Zuo 1, 5
Affiliation  

With the rapid development of nanotechnology and an increasing use of nanoenabled consumer products, there is an urgent need to develop precautionary tools to evaluate acute lung toxicity of engineered nanomaterials (ENMs). As natural pulmonary surfactant (PS) film represents the initial barrier of nano–bio interactions in the lungs, a novel in vitro experimental method, called constrained drop surfactometry (CDS), is developed to quantitatively evaluate PS inhibition caused by ENMs. The results show that at a very low concentration, four representative ENMs, including carbon nanotubes, graphene oxide, zinc oxide, and silver nanoparticles, all increase in vitro minimum surface tension of a modified natural PS, Infasurf. These in vitro results are related to the extensive alveolar collapse and inflammation observed in vivo in mice exposed to these ENMs in an intratracheal instillation model. Thus, there may be a direct correlation between in vitro surface tension increase due to PS inhibition by ENMs and in vivo lung toxicity revealed by alveolar collapse and inflammation. Compared to commonly used animal models, CDS holds great promise for the development of an animal‐free, easy‐to‐use, and low‐cost precautionary assay for the prediction of acute lung toxicity of inhaled ENMs.

中文翻译:

肺表面活性剂的生物物理评估可预测纳米材料的肺毒性。

随着纳米技术的飞速发展和纳米消费产品的日益普及,迫切需要开发预防性工具来评估工程纳米材料(ENM)的急性肺毒性。由于天然的肺表面活性剂(PS)膜代表了肺中纳米生物相互作用的初始屏障,因此开发了一种新的体外实验方法,称为约束液滴表面测量法(CDS),以定量评估由ENM引起的PS抑制作用。结果表明,在非常低的浓度下,包括碳纳米管,氧化石墨烯,氧化锌和银纳米颗粒在内的四种代表性ENM均会提高改性天然PS Infasurf的体外最小表面张力。这些体外结果与在气管内滴注模型中暴露于这些ENM的小鼠体内观察到的广泛的肺泡塌陷和炎症有关。因此,由于ENM引起的PS抑制导致的体外表面张力增加与肺泡塌陷和炎症所揭示的体内肺毒性之间可能存在直接相关性。与常用的动物模型相比,CDS在开发无动物,易于使用且低成本的预防性测定方法以预测吸入的ENM的急性肺毒性方面具有广阔的前景。
更新日期:2018-03-07
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