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What are the current solutions for interfacing supercritical fluid chromatography and mass spectrometry?
Journal of Chromatography B ( IF 3 ) Pub Date : 2018-03-07 , DOI: 10.1016/j.jchromb.2018.03.010
Davy Guillarme , Vincent Desfontaine , Sabine Heinisch , Jean-Luc Veuthey

Mass spectrometry (MS) is considered today as one of the most popular detection methods, due to its high selectivity and sensitivity. In particular, this detector has become the gold standard for the analysis of complex mixtures such as biological samples. The first successful SFC-MS hyphenation was reported in the 80’s, and since then, several ionization sources, mass analyzers and interfacing technologies have been combined. Due to the specific physicochemical properties and compressibility of the SFC mobile phase, directing the column effluent into the ionization source is more challenging than in LC. Therefore, some specific interfaces have to be employed in SFC-MS, to i) avoid (or at least limit) analytes precipitation due to CO2 decompression, when the SFC mobile phase is not anymore under backpressure control, ii) achieve adequate ionization yield, even with a low proportion of MeOH in the mobile phase and iii) preserve the chromatographic integrity (i.e. maintaining retention, selectivity, and efficiency). The goal of this review is to describe the various SFC-MS interfaces and highlight the most favorable ones in terms of reliability, flexibility, sensitivity and user-friendliness.



中文翻译:

目前用于超临界流体色谱和质谱分析接口的解决方案是什么?

由于质谱法(MS)的高选择性和灵敏度,它被认为是当今最受欢迎的检测方法之一。特别是,这种检测器已成为分析复杂混合物(例如生物样品)的金标准。自80年代以来,首次成功进行了SFC-MS断字的报道,从那时起,已将多种电离源,质量分析仪和接口技术相结合。由于SFC流动相的特定物理化学性质和可压缩性,将色谱柱流出物引入电离源比在LC中更具挑战性。因此,在SFC-MS中必须使用某些特定的界面,以i)避免(或至少限制)由于CO 2引起的分析物沉淀减压时,如果SFC流动相不再处于背压控制下,则ii)即使在流动相中的MeOH含量较低时也能获得足够的电离产率,并且iii)保留色谱完整性(即保持保留,选择性和效率)。这篇综述的目的是描述各种SFC-MS接口,并在可靠性,灵活性,灵敏度和用户友好性方面突出最有利的接口。

更新日期:2018-03-07
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