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Analysis of unauthorized Sudan dyes in food by high-performance thin-layer chromatography
Analytical and Bioanalytical Chemistry ( IF 4.3 ) Pub Date : 2018-03-07 , DOI: 10.1007/s00216-018-0945-6
Wolfgang Schwack , Elodie Pellissier , Gertrud Morlock

Food authenticity and food safety are of high importance to organizations as well as to the food industry to ensure an accurate labeling of food products. Respective analytical methods should provide a fast screening and a reliable cost-efficient quantitation. HPTLC was pointed out as key analytical technique in this field. A new HPTLC method applying caffeine-impregnated silica gel plates was developed for eight most frequently found fat-soluble azo dyes unauthorizedly added to spices, spice mixtures, pastes, sauces, and palm oils. A simple post-chromatographic UV irradiation provided an effective sample cleanup, which took 4 min for up to 46 samples in parallel. The method was trimmed to enable 23 simultaneous separations within 20 min for quantitation or 46 separations within 5 min for screening. Linear (4–40 ng/band) or polynomial (10–200 ng/band) calibrations of the eight azo dyes revealed high correlation coefficients and low standard deviations. Limits of detection and quantification were determined to be 2–3 and 6–9 ng/zone, respectively. After an easy sample extraction, recoveries of 70–120% were obtained from chili, paprika, and curcuma powder as well as from chili sauce, curry paste, and palm oil spiked at low (mainly 25–50 mg/kg) and high levels (150–300 mg/kg). For unequivocal identification, the compound in a suspect zone was eluted via a column into the mass spectrometer. This resulted in the hyphenation HPTLC-vis-HPLC-DAD-ESI-MS.

中文翻译:

高效薄层色谱法分析食品中未经授权的苏丹染料

食品的真实性和食品安全性对组织以及食品行业来说至关重要,以确保食品的准确标签。相应的分析方法应提供快速的筛选和可靠的成本效益的定量分析。指出HPTLC是该领域的关键分析技术。开发了一种新的HPTLC方法,该方法采用了咖啡因浸渍的硅胶板,用于将八种最常见的脂溶性偶氮染料未经授权地添加到香料,香料混合物,浆糊,沙司和棕榈油中。简单的色谱后紫外线照射可提供有效的样品净化效果,最多可并行处理4分钟,最多可采集46个样品。对该方法进行了调整,可以在20分钟内同时进行23次分离以进行定量分析,或在5分钟内进行46次分离以进行筛查。八种偶氮染料的线性(4–40 ng /条带)或多项式(10–200 ng /条带)校准显示出高相关系数和低标准偏差。检出限和定量限分别为2–3 ng /区和6–9 ng /区。轻松提取样品后,从辣椒,辣椒粉和姜黄粉以及低(主要为25-50 mg / kg)和高含量的辣椒酱,咖喱酱和棕榈油中回收,可回收70-120% (150-300 mg / kg)。为了明确鉴定,将可疑区域中的化合物通过色谱柱洗脱到质谱仪中。这导致了HPTLC-vis-HPLC-DAD-ESI-MS的连接。分别。轻松提取样品后,从辣椒,辣椒粉和姜黄粉以及低(主要为25-50 mg / kg)和高含量的辣椒酱,咖喱酱和棕榈油中回收,可回收70-120% (150-300 mg / kg)。为了明确鉴定,将可疑区域中的化合物通过色谱柱洗脱到质谱仪中。这导致了HPTLC-vis-HPLC-DAD-ESI-MS的连接。分别。轻松提取样品后,从辣椒,辣椒粉和姜黄粉以及低(主要为25-50 mg / kg)和高含量的辣椒酱,咖喱酱和棕榈油中回收,可回收70-120% (150-300 mg / kg)。为了明确鉴定,将可疑区域中的化合物通过色谱柱洗脱到质谱仪中。这导致了HPTLC-vis-HPLC-DAD-ESI-MS的连接。
更新日期:2018-08-10
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