A novel fluorescent probe was developed by integrating chlorinated coumarin and benzothiazolylacetonitrile and exploited for simultaneous detection of cysteine (Cys), homocysteine (Hcy), and glutathione (GSH). Featuring four binding sites and different reaction mechanisms for different biothiols, this probe exhibited rapid fluorescence turn‐on for distinguishing Cys, Hcy, and GSH with 108‐, 128‐, 30‐fold fluorescence increases at 457, 559, 529 nm, respectively, across different excitation wavelengths. Furthermore, the probe was successfully applied to the fluorescence imaging of endogenous Cys and GSH and exogenous Cys, Hcy, and GSH in living cells.

中文翻译：

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具有多个结合位点的多信号荧光探针，可同时感测半胱氨酸，高半胱氨酸和谷胱甘肽

通过整合氯化香豆素和苯并噻唑基乙腈，开发了一种新型荧光探针，并用于同时检测半胱氨酸（Cys），高半胱氨酸（Hcy）和谷胱甘肽（GSH）。该探针具有四个结合位点，并且针对不同的生物硫醇具有不同的反应机制，该探针具有快速的荧光开启能力，可区分Cys，Hcy和GSH，分别在457、559、529 nm处具有108倍，128倍，30倍的荧光增加，跨不同的激发波长。此外，该探针已成功应用于活细胞中内源性Cys和GSH以及外源性Cys，Hcy和GSH的荧光成像。