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Deep Sequencing Identified miR-193b-3p as a Positive Regulator of Autophagy Targeting Akt3 in Ctenopharyngodon idella CIK Cells During GCRV Infection Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-24 Hongyan Yu, Zheyan Chen, Yuting Liu, Yubang Shen, Lang Gui, Junqiang Qiu, Xiaoyan Xu, Jiale Li
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Ferroptosis resists intracellular Vibrio splendidus AJ01 mediated by ferroportin in sea cucumber Apostichopus japonicus Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-24 Chengyang Wang, Yangxi Xiang, Yina Shao, Chenghua Li
Ferroptosis, a kind of programmed cell death, is characterized with iron-dependent lipid ROS buildup, which is considered as an important cellular immunity in resisting intracellular bacterial infection in mammalian macrophages. In this process, lipid ROS oxidizes the bacterial biofilm to inhibit intracellular bacteria. However, the function of ferroptosis in invertebrate remains unknown. In this study
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Population-level immunologic variation in wild threespine stickleback (Gasterosteus aculeatus) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-24 Anika M. Wohlleben, Javier F. Tabima, Néva P. Meyer, Natalie C. Steinel
Wild organisms are regularly exposed to a wide range of parasites, requiring the management of an effective immune response while avoiding immunopathology. Currently, our knowledge of immunoparasitology primarily derives from controlled laboratory studies, neglecting the genetic and environmental diversity that contribute to immune phenotypes observed in wild populations. To gain insight into the immunologic
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Ferulic acid alleviates avermectin induced renal injury in carp by inhibiting inflammation, oxidative stress and apoptosis Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-24 Qiulu Li, Kaixin Ping, Yannan Xiang, , Zunhan Hu, Shujuan Liu, Xinying Guan, Mian Fu
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Autophagy mediated degradation of MITA/TBK1/IRF3 by a hnRNP family member attenuates interferon production in fish Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-19 Yanwei Zhang, Jing Cen, Haixia Wu, Wa Gao, Zhiying Jia, Mikolaj Adamek, Jun Zou
HnRNP A/B belongs to the heterogeneous nuclear ribonucleoprotein (hnRNP) family and plays an important role in regulating viral protein translation and genome replication. Here, we found that overexpression of hnRNP A/B promoted Spring viremia of carp virus (SVCV) and Cyprinid herpesvirus 3 (CyHV3) replication. Further, hnRNP A/B was shown to act as a negative regulator of type I interferon (IFN) response
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Enhancement of autophagy can alleviate oxidative stress, inflammation, and apoptosis induced by ammonia stress in yellow catfish Pelteobagrus fulvidraco Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-23 Xue Li, Shidong Wang, Muzi Zhang, Ming Li
Ammonia in aquatic environments is toxic to fish, directly impacting their growth performance and development. Activation of autophagy can facilitate intracellular component renewal and enhance an organism's adaptability to adverse environments. Therefore, this study investigates the impact of autophagy on the yellow catfish under acute ammonia stress. In this study, the yellow catfish intraperitoneally
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Possible transport routes of IgM to the gut of teleost fish Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-23 Angela Etayo, Håvard Bjørgen, Ivar Hordvik, Aina-Cathrine Øvergård
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Effects of Lactobacillus acidophilus AC on the growth, intestinal flora and metabolism of zebrafish (Danio rerio) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-20 Haoyu QIU, Ling HUANG, Hanying WANG, Chenzhi TAO, Zhiqiang RAN, Jiahang XU, Haofeng SUN, Ping WANG
The intensive aquaculture model has resulted in a heightened prevalence of diseases among farmed animals. It is imperative to identify healthy and efficacious alternatives to antibiotics for the sustainable progression of aquaculture. In this investigation, a strain of Lactobacillus acidophilus AC was introduced into the cultural water at varying concentrations (105 CFU/mL, 106 CFU/mL, 107 CFU/mL)
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The combined analysis of transcriptomics and metabolomics reveals the mechanisms by which dietary quercetin regulates growth and immunity in Penaeus vannamei Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-20 Xuanyi Yang, Baojie Wang, Keyong Jiang, Kefeng Xu, Chen Zhong, Mei Liu, Lei Wang
As a potent antioxidant, the flavonoid compound quercetin (QUE) has been widely used in the farming of aquatic animals. However, there are fewer reports of the beneficial effects, especially in improving immunity of Penaeus vannamei by QUE. The aim of this study was to investigate the effects of dietary QUE on growth, apoptosis, antioxidant and immunity of P. vannamei. It also explored the potential
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Innate immune response of snakehead fish to Indian strain of snakehead rhabdovirus (SHRV-In) infection and the infectivity potential of the virus to other freshwater fishes Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-20 Priyadharshini Michael, Sivasankar Panchavarnam, Chrisolite Bagthasingh, Subash Palaniappan, Rani Velu, Mohamed Mansoor Mohaideenpitchai, Mageshkumar Palraj, Selvamagheswaran Muthumariyapan, Evangelin Paripoorana David
A new virus known as snakehead rhabdovirus (SHRV-In) was discovered in South India in striped snakehead (Channa striata) that had hemorrhagic patches and cutaneous ulcerations. The virus is the most potentially harmful pathogen of snakehead because it could cause 100% mortality within 5 days. The goal of the current investigation was to evaluate the infectivity of rhabdovirus in freshwater fishes and
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Safety evaluation and effects of dietary phlorotannins on the growth, health, and intestinal microbiota of Litopenaeus vannamei Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-17 Zijie Wu, Wenbo Wu, Shouguo Yang, Fen Cheng, Jingyi Lv, Yingjin Shao, Xianming Tang, Erchao Li, Qun Zhao
Phlorotannins are phenolic compounds with diverse biological activities, yet their efficacy in aquatic animals currently remains unclear. This investigation scrutinized the influence of phlorotannins on the growth, immunity, antioxidant capacity, and intestinal microbiota in Litopenaeus vannamei, concurrently evaluating the potential adverse effects of phlorotannins on L. vannamei. A base diet without
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Evaluation of the efficacy of MONTANIDE™ GR01, a new adjuvant for feed-based vaccines, on the immune response and protection against Streptococcus agalactiae in oral vaccinated Nile tilapia (Oreochromis niloticus) under laboratory and on-farm conditions Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-17 Sirinya Pholchamat, Regis Vialle, Vijitra Luang-In, Panarat Phadee, Bei Wang, Tiehui Wang, Christopher J. Secombes, Eakapol Wangkahart
Streptococcosis, an emerging infectious disease caused by Streptococcus agalactiae, has had adverse effects on farmed tilapia. Several vaccines have been developed to prevent this disease and induce a specific immune response against S. agalactiae infection. In this study the use of MONTANIDE™ GR01, a new adjuvant for oral vaccination, was optimized for use in tilapia under laboratory and field studies
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Leveraging the biotechnological promise of the hagfish variable lymphocyte receptors: tools for aquatic microbial diseases Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-17 Dennis B. Bela-ong, Jaesung Kim, Kim D. Thompson, Tae Sung Jung
The jawless vertebrates (agnathans or cyclostomes) are ancestral animals comprising lampreys and hagfishes, which are the only extant representatives. They possess an alternative adaptive immune system (AIS) that uses leucine-rich repeats (LRR)-based variable lymphocyte receptors (VLRs) instead of the immunoglobulin (Ig)-based antigen receptors of jawed vertebrates (gnathostomes). The five VLR types
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Pathological Variations and Immune Response in Channa argus Infected with Pathogenic Nocardia seriolae Strain Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-17 Tingting Zhou, Ping Cai, Junwei Li, Xueming Dan, Zhongsheng Li
Nocardia seriolae pathogen causes chronic granulomatous disease, reportedly affecting over 40 species of marine and freshwater cultured fish. Hence, research is required to address and eliminate this significant threat to the aquaculture industry. In this respect, a reliable and reproducible infection model needs to be established to better understand the biology of this pathogen and its interactions
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Establishment and characterization of a golden pompano (Trachinotus blochii) fin cell line for applications in marine fish pathogen immunology Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-16 Jin-Feng Tong, Lang Yu, Rui-Hai Gan, Li-Ping Shi, Shao-Yang Bu, Yue Gu, Xin Wen, Jun-Long Sun, Fei-Biao Song, Li Zhou, Jian-Fang Gui, Jian Luo
Pompano fishes have been widely farmed worldwide. As a representative commercial marine species of the Carangidae family, the golden pompano (Trachinotus blochii) has gained significant popularity in China and worldwide. However, because of rapid growth and high-density aquaculture, the golden pompano has become seriously threatened by various diseases. Cell lines are the most cost-effective resource
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Functional characterization of NOD1 from golden pompano Trachinotus ovatus Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-16 Yadan Wang, Shaoyu Yang, Xiaohui Cai, Zhuang Huang, Karsoon Tan, Peng Xu
Fish rely on innate immune system for immunity, and nucleotide-binding oligomerization domain-like receptors (NLRs) are a vital group of receptor for recognition. In the present study, NOD1 gene was cloned and characterized from golden pompano Trachinotus ovatus, an commercially important aquaculture fish species. The ORF of T. ovatus NOD1 was 2820 bp long, encoding 939 amino acid residues with a highly
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Corrigendum to “A novel C-type lectin for Litopenaeus vannamei involved in the innate immune response against Vibrio infection” [Fish Shellfish Immunol. 135 (2023) 108621] Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-16 Junliang Luo, Yanghui Chen, Yongxiong Huang, Jiamin Feng, Yunhao Yuan, Jichang Jian, Shuanghu Cai, Shiping Yang
No Abstract
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Incorporating Lycium barbarum residue in diet boosts survival, growth, and liver health in juvenile grass carp (Ctenopharyngodon idellus) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-16 Xuewen Jia, Hongyan Yu, Biao Du, Yubang Shen, Lang Gui, Xiaoyan Xu, Jiale Li
This research elucidates the potential of Lycium barbarum residue (LBR), a by-product rich in bioactive substances, as a dietary supplement in aquaculture, especially for herbivorous fish like grass carp. In a detailed 120-day feeding trial, the impacts of varying LBR levels on juvenile grass carp were assessed, focusing on growth performance, survival rate, biochemical markers, and liver health. The
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Recombinant Lactococcal-based oral vaccine for protection against Streptococcus agalactiae infections in tilapia (Oreochromis niloticus) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-16 Kuan-Yee Wong, Megat Hamzah Megat Mazhar Khair, Adelene Ai-Lian Song, Mas Jaffri Masarudin, Jiun-Yan Loh, Chou-Min Chong, John Beardall, Michelle Yee-Mun Teo, Lionel Lian-Aun In
Streptococcosis outbreaks caused by Streptococcus agalactiae infection in tilapia aquaculture have been consistently reported and associated with high mortality and morbidity leading to significant economic losses. Existing vaccine candidates against Streptococcus spp. are designed for intraperitoneal injections that are not practical and labor-intensive which have prompted farmers to protect aquatic
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Fish Arginase constrains Excessive Production of Nitric Oxide and Limits Mitochondrial Damage during Aeromonas hydrophila infection Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-16 Yingfu Dong, Nan Wang, Hong Zhou, Xinyan Wang, Anying Zhang, Kun Yang
Bacteria-enhanced inducible nitric oxide synthase (iNOS) overproduces nitric oxide (NO) leading to mitochondrial and cellular damage. In mammals, arginase (ARG), the enzyme consuming the same substrate L-arginine with iNOS, was believed to inhibit iNOS activity by competing the substrate. But in fish, this conception has been widely challenged. In this study, the gene expression using real-time quantitative
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IL-6/STAT3 axis is hijacked by GCRV to facilitate viral replication via suppressing type Ⅰ IFN signaling Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-16 Liang Hu, Yang Xu, Qiu-Shi Zhang, Xiao-Ying Chen, Chun Li, Rui Chen, Guo-Li Hou, Zhao Lv, Tiao-Yi Xiao, Jun Zou, Hong-Quan Wang, Jun-Hua Li
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USP14 negatively regulates IFN signaling by dampening K63-linked ubiquitination of TBK1 in black carp Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-16 Can Yang, Juanjuan Shu, Xiao Yang, Yujia Miao, Ji Liu, Jun Li, Jun Xiao, Weiguang Kong, Zhen Xu, Hao Feng
USP14 regulates the immune related pathways by deubiquitinating the signaling molecules in mammals. In teleost, USP14 is also reported to inhibit the antiviral immune response through TBK1, but its regulatory mechanism remains obscure. To elucidate the role of USP14 in the RLR/IFN antiviral pathway in teleost, the homolog USP14 (bcUSP14) of black carp (Mylopharyngodon piceus) has been cloned and characterize
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Toll-interacting protein is activated by the transcription factor GATA1 and Sp1 to negatively regulate NF-κB and MAPK pathways in the Japanese eel (Anguilla japonica) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-16 Tianyu Wang, Hui Ge, Peng Lin, Yilei Wang, Xiaojian Lai, Pengyun Chen, Fuyan Li, Jianjun Feng
Toll-interacting protein (Tollip) serves as a crucial inhibitory factor in the modulation of Toll-like receptor (TLR)-mediated innate immunological responses. The structure and function of Tollip have been well documented in mammals, yet the information in teleost remained limited. This work employed in vitro overexpression and RNA interference in vivo and in vitro to comprehensively examine the regulatory
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Histone derived antimicrobial peptides identified from Mytilus coruscus serum by peptidomics Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-15 Haodong Wang, Zhi Liao, Zongxin Yang, Wenhui Xiao, Zilin Yang, Jianyu He, Xiaolin Zhang, Xiaojun Yan, Changsheng Tang
Histones and their N-terminal or C-terminal derived peptides have been studied in vertebrates and presented as potential antimicrobial agents playing important roles in the innate immune defenses. Although histones and their derived peptides had been reported as components of innate immunity in invertebrates, the knowledge about the histone derived antimicrobial peptides (HDAPs) in invertebrates are
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Tissue factor pathway inhibitors disrupt structures of rhabdovirus/ranairidovirus and inhibit viral infection in Chinese perch, Siniperca chuatsi Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-13 Baofu Ma, Jingkang Li, Min Zhang, Xiaozhe Fu, Hongru Liang, Yinjie Niu, Qiang Lin, Xia Luo, Lihui Liu, Jianguo Su, Jin Zhou, Ningqiu Li
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Corrigendum to “Infectious hypodermal and hematopoietic necrosis virus-like particle (IHHNV-VLP) induces peroxiredoxin expression and activity in Fenneropenaeus merguiensis” [Fish Shellfish Immunol. 121 (2022) 53–61] Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-12 Wattana Weerachatyanukul, Chettupon Pooljun, Ikuo Hirono, Hidehiro Kondo, Charoonroj Chotwiwatthanakun, Pitchanee Jariyapong
No Abstract
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Effects of essential amino acids supplementation in a low-protein diet on growth performance, intestinal health and microbiota of juvenile blotched snakehead (Channa maculata) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-12 Shuzhan Fei, Jiamin Kang, Mi Ou, Haiyang Liu, Xincheng Zhang, Qing Luo, Kaibin Li, Kunci Chen, Jian Zhao
Developing a low-protein feed is important for the sustainable advancement of aquaculture. The aim of this study was to investigate the effects of essential amino acid (EAA) supplementation in a low-protein diet on the growth, intestinal health, and microbiota of the juvenile blotched snakehead, Channa maculata in an 8-week trial conducted in a recirculating aquaculture system. Three isoenergetic diets
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Molecular confirmation and functional study of signal transducer and activator of transcription genes in the Pacific oyster Crassostrea gigas Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-12 Jilv Ma, Jie Cui, Yifan Qu, Jiwen Chen, Yue Zhao, Yijing Han, Yaqiong Liu, Baoyu Huang, Xiaotong Wang
The JAK (Janus kinase)-STAT (Signal transducer and activator of transcription) is a well-known functional signaling pathway that plays a key role in several important biological activities such as apoptosis, cell proliferation, differentiation, and immunity. However, limited studies have explored the functions of STAT genes in invertebrates. In the present study, the gene sequences of two STAT genes
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A novel vaccination strategy against Vibrio harveyi infection in Asian seabass (Lates calcarifer) with the aid of oxygen nanobubbles and chitosan Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-10 Nguyen Giang Thu Lan, Ha Thanh Dong, Nguyen Tien Vinh, Krishna R. Salin, Saengchan Senapin, Khaettareeya Pimsannil, Sophie St-Hilaire, Andrew P. Shinn, Channarong Rodkhum
Immersion vaccination, albeit easier to administer than immunization by injection, sometimes has challenges with antigen uptake, resulting in sub-optimal protection. In this research, a new strategy to enhance antigen uptake of a heat-inactivated Vibrio harveyi vaccine in Asian seabass (Lates calcarifer) using oxygen nanobubble-enriched water (ONB) and positively charged chitosan (CS) was explored
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Effects of adding Bacillus subtilis natto NTU-18 in paste feed on growth, intestinal morphology, gastrointestinal microbiota diversity, immunity, and disease resistance of Anguilla japonica glass eels Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-10 Yen-Ting Lin, Yu-Chen Hung, Li-Han Chen, Kung-Ta Lee, Yu-San Han
Japanese eel, Anguilla japonica, holds significant importance in Taiwanese aquaculture. With the intensification of eel farming, the impact of Edwardsiella tarda has become increasingly severe. Consequently, the abusive use of antibiotics has risen. Bacillus subtilis natto NTU-18, a strain of Bacillus with a high survival rate in feed processing, plays a crucial role in promoting intestinal health
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Identification and functional analysis of perforin 1 from largemouth bass (Micropterus salmoides) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-09 Xiaoman Hu, Bin Li, Bowen Lu, Hui Yu, Yang Du, Jiong Chen
In this study, we present the first cloning and identification of perforin (MsPRF1) in largemouth bass (Micropterus salmoides). The full-length cDNA of MsPRF1 spans 1572 base pairs, encoding a 58.88 kDa protein consisting of 523 amino acids. Notably, the protein contains MACPF and C2 structural domains. To evaluate the expression levels of MsPRF1 in various healthy largemouth bass tissues, real-time
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Effect of dietary heat-killed Lactiplantibacillus plantarum VSG3 on growth, immunity, antioxidant status, and immune-related gene expression in pathogen-aggravated Cyprinus carpio Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-08 Sib Sankar Giri, Won Joon Jung, Sung Bin Lee, Su Jin Jo, Mae Hyun Hwang, Jae Hong Park, Sukumaran Venkatachalam, Se Chang Park
Heat-killed probiotics offer an alternative approach to enhance growth and disease resistance in farmed fish. In this study, we isolated Lactiplantibacillus plantarum VSG3 from the gut of Labeo rohita to investigate the effects of heat-killed L. plantarum (HK-LP) on the health and growth performance of Cyprinus carpio fingerlings. Different concentrations of HK-LP (0, 50, 100, 200, 300, and 400 mg/kg)
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Mucosal barrier status in Atlantic salmon fed rapeseed oil and Schizochytrium oil partly or fully replacing fish oil through winter depression Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-08 SM Majharul Islam, Florence Perera Willora, Mette Sørensen, Golam Rbbani, Muhammad A.B. Siddik, Kyla Zatti, Shruti Gupta, Ian Carr, Ester Santigosa, Monica F. Brinchmann, Kim D. Thompson, Ioannis N. Vatsos
The study was designed to investigate the effects of replacing fish oil by algal oil and rapeseed oil on histomorphology indices of the intestine, skin and gill, mucosal barrier status and immune-related genes of mucin and antimicrobial peptide (AMP) genes in Atlantic salmon (Salmo salar). For these purposes, Atlantic salmon smolts were fed three different diets. The first was a control diet containing
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Pleurotus eryngii root waste and soybean meal co-fermented protein improved the growth, immunity, liver and intestinal health of largemouth bass (Micropterus salmoides) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-08 Jian-Ming Xu, Wen-Rong Gao, Ping Liang, Guo-He Cai, Hong-Ling Yang, Jian-Bin Lin, Yun-Zhang Sun
The present study aimed to evaluate the effect of king oyster mushroom (Pleurotus eryngii) root waste and soybean meal co-fermented protein (CFP) on growth performance, feed utilization, immune status, hepatic and intestinal health of largemouth bass (Micropterus salmoides). Largemouth bass (12.33 ± 0.18 g) were divided into five groups, fed with diets containing 0 %, 5 %, 10 %, 15 % and 20 % CFP respectively
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Antiviral effect of miR-206 in rainbow trout (Oncorhynchus mykiss) against infectious hematopoietic necrosis virus infection Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-08 Shenji Wu, Jinqiang Huang, Yongjuan Li
Infectious hematopoietic necrosis (IHN), caused by IHN virus, is a highly contagious and lethal disease that seriously hampers the development of rainbow trout (Oncorhynchus mykiss) aquaculture. However, the immune response mechanism of rainbow trout underlying IHNV infection remains largely unknown. MicroRNAs act as post-transcriptional regulators of gene expression and perform a crucial role in fish
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STAM2 negatively regulates the MyD88-mediated NF-κB signaling pathway in miiuy croaker, Miichthys miiuy Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-07 Yan Xu, Wenxin Li, Ya Chen, Tianjun Xu, Yuena Sun
Signal transducing adapter molecule 2 (STAM2), a member of the Signal Transducing Adapter Molecule (STAM) family, is a protein with significant implications in diverse signaling pathways and endocytic membrane trafficking. However, the role of the STAM2, especially in fish, remains largely unknown. In this study, we discovered that STAM2 negatively regulates the NF-κB signaling pathway, and its inhibitory
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Molecular characterization of a short-chained pentraxin gene from kuruma shrimp Marsupenaeus japonicus hemocytes Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-07 Omar Adrianne P. Alaman, Ivane R. Pedrosa-Gerasmio, Keichiro Koiwai, Reiko Nozaki, Hidehiro Kondo, Ikuo Hirono
Pentraxins (PTXs) are a family of pattern recognition proteins (PRPs) that play a role in pathogen recognition during infection via pathogen-associated molecular patterns (PAMPs). Here, we characterized a short-chained pentraxin isolated from kuruma shrimp (Marsupenaeus japonicus) hemocytes (MjPTX). MjPTX contains the pentraxin signature HxCxS/TWxS (where x can be any amino acid), although the second
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A novel exoskeletal-derived C-type lectin facilitates phagocytosis of hemocytes in the oriental river prawn Macrobrachium nipponense Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-04 Juan Du, Yuanyuan Tang, Jiaye Chu, Quanli Yang, Xiaohan Qian, Yan Wan, Yuming Lu, Limin Zhang, Wenfeng Wang
C-type lectins (CTLs) execute critical functions in multiple immune responses of crustaceans as a member of pattern recognition receptors (PRRs) family. In this study, a novel CTL was identified from the exoskeleton of the oriental river prawn Macrobrachium nipponense (MnLec3). The full-length cDNA of MnLec3 was 1150 bp with an open reading frame of 723 bp, encoding 240 amino acids. MnLec3 protein
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Aggregation and proliferation of B cells and T cells in MALTs upon Cryptocaryon irritans infection in large yellow croaker Larimichthys crocea Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-04 Yangyang Ding, Yameng Zhang, Yibo Shen, Yihan Zhang, Zhangqi Li, Yuan Shi, Zhengwei Cui, Xinhua Chen
Mucosal immunity in mucosa-associated lymphoid tissues (MALTs) plays crucial roles in resisting infection by pathogens, including parasites, bacteria and viruses. However, the mucosal immune response in the MALTs of large yellow croaker (Larimichthys crocea) upon parasitic infection remains largely unknown. In this study, we investigated the role of B cells and T cells in the MALTs of large yellow
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The combination of high temperature and Vibrio infection worsens summer mortality in the clam Meretrix petechialis by increasing apoptosis and oxidative stress Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-03 Jing Tian, Di Wang, Hongxia Wang, Pin Huan, Baozhong Liu
The interaction between environmental factors and Vibrio in bivalves is not well understood, despite the widely held belief that pathogen infection and seawater temperature significantly impact summer mortality. In the present study, we conducted simulated experiments to explore the effects of high temperature and Vibrio infection on the clam Meretrix petechialis. The survival curve analysis revealed
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Identification, diversity, and evolution analysis of Commd gene family in Haliotis discus hannai and immune response to biotic and abiotic stresses Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-02 Yuping Chen, Wenxin Zhang, Xin Chen, Tao Zhang, Huina Wei, Jianpeng Huang, Chao Fan, Mingyi Cai, Yilei Wang, Ziping Zhang
The Commd (Copper Metabolism gene MURR1 Domain) family genes play crucial roles in various biological processes, including copper and sodium transport regulation, NF-κB activity, and cell cycle progression. Their function in Haliotis discus hannai, however, remains unclear. This study focused on identifying and analyzing the Commd genes in H. discus hannai, including their gene structure, phylogenetic
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Effects of supplementation with different zinc-based products on the growth and health of Nile tilapia Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-02 Vitor Fernandes Silva, Marília Tedesco, Silvia Terra Fontes, Marco Shizuo Owatari, Yuri Malaquias Gauglitz Gatto, Matheus Berlofa Ferreira, Paola Capistrano dos Santos, Gabriel Antonio Cuzma Costa, Adriano Faria Palmieri, Gracienhe Gomes dos Santos, Miguel Saldaña-Serrano, Afonso Celso Dias Bainy, Maurício Laterça Martins, José Luiz Pedreira Mouriño
Zinc is one of the essential microelements for the metabolism of animals. Zinc nanoparticles may have higher bioavailability due to their low specific surface area, facilitating absorption by fish. The present study aimed to evaluate the effects of supplementation with different zinc-based products on the growth and health of Nile tilapia Oreochromis niloticus. Zinc, in different sizes (nanoparticles
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Study of the antivirus function mediated by STING in Micropterus salmoides Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-02 Jin-Quan Fan, Qian-Ming Hong, Li-Shi Liu, Qi Chen, Yi-Hong Chen
Stimulator of interferon genes (STING) has been demonstrated as a critical mediator in the innate immune response to cytosolic DNA and RNA derived from different pathogens. While the role of Micropterus salmoides STING (MsSTING) in largemouth bass virus is still unknown. In this study, RT-qPCR assay and Western-blot assay showed that the expression levels of MsSTING and its downstream genes were up-regulated
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Fish ELOVL7a is involved in virus replication via lipid metabolic reprogramming Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-04-01 Qi Zheng, Lin Liu, Xixi Guo, Fengyi Zhu, Youhua Huang, Qiwei Qin, Xiaohong Huang
The elongation of very long chain fatty acids (ELOVL) proteins are key rate-limiting enzymes that catalyze fatty acid synthesis to form long chain fatty acids. ELOVLs also play regulatory roles in the lipid metabolic reprogramming induced by mammalian viruses. However, little is known about the roles of fish ELOVLs during virus infection. Here, a homolog of ELOVL7 was cloned from Epinephelus coioides
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Okadaic acid enhances NfKB, TLR-4, caspase 3, ERK ½, c-FOS, and 8-OHdG signaling pathways activation in brain tissues of zebrafish larvae Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-03-30 Ahmet Topal, Hatice Oğuş, Ekrem Sulukan, Selim Comaklı, Saltuk Buğrahan Ceyhun
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Effects of Citrobacter freundii on sturgeon: Insights from skin mucosal immunology and microbiota Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-03-30 Jiehao Liu, Yiting Pan, Shen Jin, Yueping Zheng, Jianan Xu, Houyong Fan, Mansoor Khalid, Youji Wang, Menghong Hu
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Exploration of the immune response of grass carp (Ctenopharyngodon idellus) erythrocytes during bacterial infection Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-03-28 Shiyi Yang, Yanhan Bai, Junjie Tao, Chengming Tu, Bing Chen, Xiaoman Huang, Linpeng Zhang, Lihan Liu, Lin Li, Zhendong Qin
In teleost blood, red blood cells (RBCs) are the most common type of cell, and they differ from mammalian RBCs in having a nucleus and other organelles. As nucleated cells, teleost RBCs contribute to the immune response against pathogens, but their antibacterial mechanism remains unclear. Here, we utilized RNA-Seq to analyze gene expression patterns of grass carp (Ctenopharyngodon idellus) RBCs (GcRBCs)
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Berberine inhibits SGIV replication by suppressing inflammatory response and oxidative stress Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-03-26 Yunxiang Jiang, Chengzong Han, Hannan Gong, Jiatao Chen, Biao Tang, Min Yang, Qiwei Qin, Shina Wei
Singapore grouper iridovirus (SGIV) is one of the major infectious diseases responsible for high mortality and huge economic losses in the grouper aquaculture industry. Berberine (BBR), a naturally occurring plant alkaloid, is a phytochemical having a variety of biological properties, such as antiviral, antioxidant, and anti-inflammatory effects. In this work, we used an in vitro model based on Western
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MicroRNA-30e-3p regulates the inflammatory response by targeting the gimap8 gene in Ctenopharyngodon idella kidney (CIK) cells Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-03-23 Lingli Xie, Biao Du, Hongyan Yu, Lang Gui, Junqiang Qiu, Li Yuan, Yubang Shen, Xiaoyan Xu, Jiale Li
Recent studies have increasingly linked miRNAs with the modulation of inflammatory responses and immunosuppressive activities. This investigation reveals that mir-30e-3p selectively binds to and modulates gimap8, as demonstrated by luciferase reporter assays and qPCR analyses. Upon LPS stimulation of CIK cells, mir-30e-3p expression was notably elevated, inversely correlating with a decrease in gimap8
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FerrylHb induces inflammation and cell death in grass carp (Ctenopharyngodon idella) hepatocytes Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-03-19 Junjie Tao, Lihan Liu, Xiaoman Huang, Chenming Tu, Linpeng Zhang, Shiyi Yang, Yanhan Bai, Lin Li, Zhendong Qin
Grass carp hemorrhagic disease is a significant problem in grass carp aquaculture. It releases highly oxidizing hemoglobin (Hb) into tissues, induces rapid autooxidation, and subsequently discharges cytotoxic reactive oxygen species (ROS). However, the mechanism underlying Hb damage to the teleost remains unclear. Here, we employed ferrylHb and heme to incubate L8824 (grass carp liver) cells and quantitatively
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Molecular characterization, antibacterial and immunoregulatory activities of liver-expressed antimicrobial peptide 2 in black rockfish, Sebastes schlegelii Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-28 Min Zhang, Xue Yan, Chang-biao Wang, Wen-qing Liu, Yue Wang, Hao Jing, Bing Wang, Kai Yang, Zi-yue Chen, Yu-yu Luan, Guang-hua Wang
LEAP2 (liver expression antimicrobial peptide 2), is an antimicrobial peptide widely found in vertebrates and mainly expressed in liver. LEAP2 plays a vital role in host innate immunity. In teleosts, a number of LEAP2 homologs have been reported, but their effects on host defense are still limited. In this study, a LEAP2 homolog () was identified from black rockfish, , and its structure, expression
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Extracellular ATP- and adenosine-mediated purinergic signaling modulates inducible nitric oxide synthase (iNOS) gene expression, enzyme activity and nitric oxide production in common carp (Cyprinus carpio) head kidney macrophages Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-27 Shuo Li, Xiwen Cui, Yue Cao, Jinsheng Sun
Inducible nitric oxide (NO) synthase (iNOS) is a key immune mediator for production of inflammatory mediator NO from -arginine. Tight regulation of iNOS expression and enzyme activity is critical for proper NO productions under inflammation and infection conditions. However, the regulatory mechanism for iNOS expression and enzyme activity in fish remains largely unknown. Here, we show that extracellular
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Biocompatibility of intraperitoneally implanted TEMPO-oxidized cellulose nanofiber hydrogels for antigen delivery in Atlantic salmon (Salmo salar L.) vaccines Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-25 Sarah M. Turner, Kora Kukk, Inga F. Sidor, Michael D. Mason, Deborah A. Bouchard
Disease outbreaks are a major impediment to aquaculture production, and vaccines are integral for disease management. Vaccines can be expensive, vary in effectiveness, and come with adjuvant-induced adverse effects, causing fish welfare issues and negative economic impacts. Three-dimensional biopolymer hydrogels are an appealing new technology for vaccine delivery in aquaculture, with the potential
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Bioinformatics characteristics and expression analysis of IL-8 and IL-10 in largemouth bass (Micropterus salmoides) upon Nocardia seriolae infection Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-25 Qin Wang, Zhang chun Zhao, Ruhan Sun, Zechao Shi, Yaqian Zhang, Bingchao Wang, Xuezhen Zhang, Wei Ji
IL-8 and IL-10 are crucial inflammatory cytokines that participate in defending host cells against infections. To demonstrate the function of the two interleukin genes in largemouth bass (), we initially cloned and identified the cDNA sequences of and in largemouth bass, referred to as and , respectively. The open reading frame (ORF) of was 324 bp in length, encoding 107 amino acids, while the ORF
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Dietary methanotroph bacteria meal alleviates soybean meal-induced enteritis by improving immune tolerance and intestinal flora profile of juvenile turbot (Scophthalmus maximus L.) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-23 Jichang Zheng, Wencong Zhang, Zhijie Dan, Xiufei Cao, Ye Gong, Kangsen Mai, Qinghui Ai
An 8-week growth trial was performed to investigate the protective effects of methanotroph bacteria meal (MBM) produced from methane against soybean meal-induced enteritis (SBMIE) in juvenile turbot ( L.). Five isonitrogenous and isolipidic diets were formulated: fishmeal-based diet (FM, the control group); FM with approximate 50% of fishmeal substituted by 399.4 g/kg soybean meal (SBM); SBM supplemented
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Functional identification of two HMGB1 paralogues provides insights into autophagic machinery in teleost Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-21 Dan Wang, Xiaoyu Ma, Hengyi Hu, Jingqi Ren, Jiaxi Liu, Hong Zhou
High mobility group box 1 (HMGB1) is a multifunctional regulator that plays different roles in various physiological and pathological processes including cell development, autophagy, inflammation, tumor metastasis, and cell death based on its cellular localization. Unlike mammalian HMGB1, two HMGB1 paralogues (HMGB1a and HMGB1b) have been found in fathead minnow and other fish species and its function
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Effects of different concentrations and particle sizes of nanoplastics on gut microbiology, metabolism, and immunity in Chiromantes dehaani Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-20 Mingming Han, Tian Zhu, Zihan Zhou, Qin Si, Chenxi Zhu, Yiming Li, Qichen Jiang
This study investigated the effects of nanoplastics (NPs) of varying particle sizes (75, 500, and 1000 nm) and concentrations (2.5 and 10 mg/L) on the gut health of . The experimental groups included a control (Cg0), and varying combinations of particle size and concentration. Our results showed that 75 nm NPs were more likely to enhance pathogenic bacterial growth than other sized NPs. Compared with
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Damage of polyethylene microplastics on the intestine multilayer barrier, blood cell immune function and the repair effect of Leuconostoc mesenteroides DH in the large-scale loach (Paramisgurnus dabryanus) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-19 Xiaohua Xia, Xiaoyu Ma, Ning Liang, Lu Qin, Weiran Huo, Yi Li
Polyethylene microplastics (PE-MPs) has become a global concern due to their widespread distribution and hazardous properties in aquatic habitats. In this study, the accumulation effect of PE-MPs in the intestine of large-scale loach () was explored by adding different concentrations of PE-MPs to the water, the destination of PE-MPs after breaking the intestinal barrier and the effects caused. The
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Transcriptomic analysis of turbot (Scophthalmus maximus) treated with zymosan a reveals that lncRNAs and inflammation-related genes mediate the protection conferred against Aeromonas salmonicida Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-17 Alejandro Romero, Magalí Rey-Campos, Patricia Pereiro, Marta Librán-Pérez, Antonio Figueras, Beatriz Novoa
is one of the most harmful pathogens in finfish aquaculture worldwide. Immunostimulants such as β-glucans are used to enhance the immunity of cultured fish. However, their effects on fish physiology are not completely understood. In the present work, we evaluated the effect of a single intraperitoneal (ip) injection of zymosan A on fish survival against infection. A single administration of this compound
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A comprehensive review on the utilization of probiotics in aquaculture towards sustainable shrimp farming Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-17 C.M. Mathan Muthu, A.S. Vickram, B. Bhavani Sowndharya, A. Saravanan, R. Kamalesh, Yuvaraj Dinakarkumar
Probiotics in shrimp aquaculture have gained considerable attention as a potential solution to enhance production efficiency, disease management, and overall sustainability. Probiotics, beneficial microorganisms, have shown promising effects when administered to shrimp as dietary supplements or water additives. Their inclusion has been linked to improved gut health, nutrient absorption, and disease