Time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to study the effects of cocaine versus methylphenidate administration on both the localization and abundance of lipids in Drosophila melanogaster brain. A J105 ToF-SIMS with a 40 keV gas cluster primary ion source enabled us to probe molecular ions of biomolecules on the fly with a spatial resolution of ∼3 μm, giving us unique insights into the effect of these drugs on molecular lipids in the nervous system. Significant changes in phospholipid composition were observed in the central brain for both. Principal components image analysis revealed that changes occurred mainly for phosphatidylcholines, phosphatidylethanolamines, and phosphatidylinositols. When the lipid changes caused by cocaine were compared with those induced by methylphenidate, it was shown that these drugs exert opposite effects on the brain lipid structure. We speculate that this might relate to the molecular mechanism of cognition and memory.

中文翻译：

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质谱成像显示可卡因和哌醋甲酯对果蝇脑中的主要脂质具有相反的作用

飞行时间二次离子质谱法（ToF-SIMS）用于研究可卡因与哌醋甲酯给药对黑腹果蝇脂质定位和脂质富集的影响脑。具有40 keV气体簇主离子源的J105 ToF-SIMS使我们能够以约3μm的空间分辨率在飞行中探测生物分子的分子离子，从而使我们对这些药物对神经分子脂质的作用具有独特的见解。系统。两者在中央大脑中均观察到磷脂成分的显着变化。主成分图像分析表明，变化主要发生在磷脂酰胆碱，磷脂酰乙醇胺和磷脂酰肌醇上。当将可卡因引起的脂质变化与哌醋甲酯引起的脂质变化进行比较时，表明这些药物对脑脂质结构具有相反的作用。我们推测这可能与认知和记忆的分子机制有关。